Ushijima H, Kunisada T, Ami Y, Tsuchie H, Takahashi I, Schäcke H, Müller W E
Division of AIDS Virus, National Institute of Health, Tokyo, Japan.
Pathobiology. 1993;61(3-4):145-53. doi: 10.1159/000163783.
The myeloid-monocytic cells ML-1, HL-60, THP-1 and U-937 were chronically infected (for more than 2 years) with the lymphotropic HIV-1 strain HTLV-IIIB. Reinfection experiments revealed that viruses obtained from chronically infected ML-1/HIV-1 and HL-60/HIV-1 cells show a low infectivity if tested with uninfected ML-1 and HL-60 cells in contrast to virus preparations from chronically infected THP-1/HIV-1 and U-937/HIV-1 with their corresponding uninfected cell lines. Analyses of selected cell surface markers showed a differential expression of CD4, CD8, CD11c, CD14, CD15, CD20, HLA-DR and HLA-DQ in non- or chronically infected cells. During chronical infection, the myeloid-monocytic cells lost their reactivity with peroxidase and esterase. In chronically infected cells, the steady-state levels for TNF-alpha mRNA remained unchanged while those for IL-6 decreased. The half-lives of transcripts of both TNF-alpha (t1/2: 70 min) and IL-6 (t1/2: 100 min) were nearly the same in uninfected and chronically infected HL-60 cells.
髓单核细胞ML-1、HL-60、THP-1和U-937被嗜淋巴细胞性HIV-1毒株HTLV-IIIB慢性感染（超过2年）。再感染实验表明，从慢性感染的ML-1/HIV-1和HL-60/HIV-1细胞中获得的病毒，与从慢性感染的THP-1/HIV-1和U-937/HIV-1及其相应未感染细胞系中获得的病毒制剂相比，在用未感染的ML-1和HL-60细胞进行测试时显示出低感染性。对选定细胞表面标志物的分析显示，在未感染或慢性感染的细胞中，CD4、CD8、CD11c、CD14、CD15、CD20、HLA-DR和HLA-DQ存在差异表达。在慢性感染期间，髓单核细胞失去了与过氧化物酶和酯酶的反应性。在慢性感染的细胞中，TNF-α mRNA的稳态水平保持不变，而IL-6的稳态水平则下降。在未感染和慢性感染的HL-60细胞中，TNF-α（半衰期：70分钟）和IL-6（半衰期：100分钟）转录本的半衰期几乎相同。
