López S, Padilla-Noriega L, Arias C F
Departamento de Biología Molecular, Universidad Nacional Autónoma de México, Cuernavaca, Morelos.
Bol Med Hosp Infant Mex. 1993 Oct;50(10):736-40.
In order to study the correlation between the serotype specificity and the genomic RNA electrophoretic pattern (electropherotype) of human rotavirus (HRV) strains, we analyzed the electropherotypes of 54 HRV that had been collected during a four year study in Mexico, and whose serotypes had been previously determined. We detected 17 different electropherotypes, four in association with serotype G1, two with serotype G2, six with serotype G3, and five with serotype G4. There were no viruses with the same electropherotype having a different serotype. The variations in RNA electrophoretic migration were greater between viruses belonging to different serotypes than between viruses of the same serotype. It is of note that the relative separation of RNA segments 7 and 9 remained constant among viruses of the same serotype. Electropherotyping might have a serotype predictive value for rotavirus specimens lacking the virion outer capsid.
为了研究人类轮状病毒(HRV)毒株的血清型特异性与基因组RNA电泳图谱(电泳型)之间的相关性,我们分析了在墨西哥进行的一项为期四年的研究中收集的54株HRV的电泳型,这些毒株的血清型此前已确定。我们检测到17种不同的电泳型,其中四种与G1血清型相关,两种与G2血清型相关,六种与G3血清型相关,五种与G4血清型相关。不存在具有不同血清型但电泳型相同的病毒。不同血清型病毒之间RNA电泳迁移的差异大于同一血清型病毒之间的差异。值得注意的是,同一血清型病毒中RNA片段7和9的相对分离保持恒定。对于缺乏病毒粒子外衣壳的轮状病毒标本,电泳分型可能具有血清型预测价值。