el-Assouli S M
Department of Medical Biology, School of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia.
J Diarrhoeal Dis Res. 1996 Sep;14(3):201-6.
In an epidemiological study of human rotavirus (HRV) infections in metro Jeddah, Saudi Arabia, the relationships among subgroups, serotypes, and RNA electropherotypes of the rotavirus isolates were investigated. Of the 523 rotavirus-positive stool specimens, 245 were examined for subgroup, serotype, and electropherotype. Of these, 84 isolates were analyzed for their subgroup and RNA electropherotype specificites, 12 (14.3%) were of subgroup 1, 69 (82.1%) were of subgroup II, and 3 (3.6%) were a mixture of subgroup I and II. Of the subgroup 1 specimens, 5 (41.7%) showed long electrophoretic migration patterns and 7 (58.3%) showed short patterns. In subgroup II specimens, 66 (95.7%) were of long patterns and 3 (4.3%) of short patterns. The relationship between HRVS serotypes and electropherotypes was also determined for the same 245 rotavirus specimens. Of these, 36 (14.7%) exhibited short RNA patterns and 209 (85.3%) exhibited long patterns. The short pattern specimens consisted of serotype 1 (8.3%), serotype 2 (63.9%), serotype 3 and serotype 4 (2.8%) each. The long pattern specimens consisted of serotype 1 (60.3%), serotype 2 (1.4%), serotype 3 (7.2%) and serotype 4 (17.7%). Among the previous 245 specimens, subgroup specificities were available for 51 specimens. All subgroup I were of serotype 2, and all subgroup II were of serotype 1, 3 or 4. RNAs of either subgroup showed both long and short electropherotypes. No relationship could be established between subgroups or serotypes and a particular electropherotype. It seems unlikely that electropherotyping of human rotavirus (HRV) can be used for identifying the subgroups or serotypes of strains.
在沙特阿拉伯吉达市地铁区域进行的一项关于人类轮状病毒（HRV）感染的流行病学研究中，调查了轮状病毒分离株的亚组、血清型和RNA电泳型之间的关系。在523份轮状病毒阳性粪便标本中，对245份进行了亚组、血清型和电泳型检测。其中，对84份分离株进行了亚组和RNA电泳型特异性分析，12份（14.3%）为I亚组，69份（82.1%）为II亚组，3份（3.6%）为I亚组和II亚组的混合。在I亚组标本中，5份（41.7%）呈现长电泳迁移模式，7份（58.3%）呈现短模式。在II亚组标本中，66份（95.7%）为长模式，3份（4.3%）为短模式。对同样的245份轮状病毒标本还确定了HRV血清型和电泳型之间的关系。其中，36份（14.7%）呈现短RNA模式，209份（85.3%）呈现长模式。短模式标本包括血清型1（8.3%）、血清型2（63.9%）、血清型3和血清型4各（2.8%）。长模式标本包括血清型1（60.3%）、血清型2（1.4%）、血清型3（7.2%）和血清型4（17.7%）。在之前的245份标本中，51份有亚组特异性信息。所有I亚组均为血清型2，所有II亚组均为血清型1、3或4。两个亚组的RNA均呈现长和短电泳型。在亚组或血清型与特定电泳型之间无法建立关系。人类轮状病毒（HRV）的电泳分型似乎不太可能用于鉴定毒株的亚组或血清型。
