Wang J B
Department of otorhinolaryngology, Union Hospital, Tong-Ji Medical University, Wuhan.
Zhonghua Er Bi Yan Hou Ke Za Zhi. 1993;28(3):149-50, 186-7.
For many years in our laboratory, toads red blood cells have been used as biomicrospheres for study of cochlear microcirculation. In the present experiments the cochlear blood flows (CBF) was twice determined in the same animal by using colored biomicrospheres. The red blood cells were stained separately with eosin and azure blue. The biomicrospheres of two different colors were injected intracardially in the same guinea pig at two different times with an interval of 4 min. The CBF were detected by using a laser Doppler system simultaneously. The finding showed that color of red and blue biomicrospheres were contrast sharply, they were all distinguished easily from the surrounding tissue. The adequate number of microspheres injected each time was 9 x 10(6)/ml. The results of CBF were not different between the first and second injection.
多年来，在我们实验室中，蟾蜍红细胞一直被用作生物微球来研究耳蜗微循环。在本实验中，使用彩色生物微球在同一只动物身上两次测定耳蜗血流量（CBF）。红细胞分别用伊红和天蓝染色。两种不同颜色的生物微球在两只不同的豚鼠体内以4分钟的间隔心内注射。同时使用激光多普勒系统检测CBF。结果表明，红色和蓝色生物微球颜色对比鲜明，它们都很容易与周围组织区分开来。每次注射的微球数量合适为9×10⁶/ml。第一次和第二次注射的CBF结果没有差异。
