Hoyland J A, Newson L, Jayson M I, Freemont A J
Department of Rheumatology, University of Manchester, U.K.
Br J Dermatol. 1993 Oct;129(4):384-8. doi: 10.1111/j.1365-2133.1993.tb03162.x.
In systemic sclerosis (SS) changes in the dermal microvasculature include endothelial cell damage, a reduction in the number of vessels, and vascular basement membrane thickening. The basement membrane is a critical component of the vessel, and alterations in its structure may lead to changes in the surrounding tissue. In SS the altered basement membrane is associated with the subsequent development of fibrosis. To investigate the relationship between vascular basement membrane changes in affected skin and disease progression, immunohistochemical analyses were performed using both polyclonal and monoclonal antibodies against type IV collagen, the major basement membrane collagen. Using two monoclonal antibodies directed against different conformational epitopes within the (alpha 1)2 (alpha 2) helical domain, type IV collagen was detected in normal skin, and uninvolved SS skin, but not in later grades of disease. Identical results were obtained using a monoclonal antibody against a sequential determinant on the denatured alpha 1 (IV) chain. The use of a polyclonal antibody, however, showed that type IV collagen was present in all grades of disease, suggesting an alteration in the composition of type IV collagen with disease progression.
在系统性硬化症（SS）中，真皮微血管的变化包括内皮细胞损伤、血管数量减少以及血管基底膜增厚。基底膜是血管的关键组成部分，其结构改变可能导致周围组织发生变化。在系统性硬化症中，改变的基底膜与随后的纤维化发展相关。为了研究受累皮肤中血管基底膜变化与疾病进展之间的关系，使用针对IV型胶原（主要的基底膜胶原）的多克隆抗体和单克隆抗体进行了免疫组织化学分析。使用两种针对（α1）2（α2）螺旋结构域内不同构象表位的单克隆抗体，在正常皮肤和未受累的系统性硬化症皮肤中检测到了IV型胶原，但在疾病后期未检测到。使用针对变性α1（IV）链上连续决定簇的单克隆抗体也得到了相同的结果。然而，使用多克隆抗体显示IV型胶原存在于所有疾病分级中，提示随着疾病进展IV型胶原的组成发生了改变。
