Sperb R A, Arnold W, Bahr G F, Löning T, Gebbers J O
Institute of Pathology, Kantonsspital Luzern, Switzerland.
Anal Cell Pathol. 1993 Sep;5(5):265-75.
Histologic sections, imprint preparations and cell preparations by enzymatic lysis from thick sections of cases of breast carcinomas were used to study total nuclear DNA distribution by image cytometry. Histologic sections are a prerequisite to unequivocally identify tumour cells as well as whole connective tissue cells used as a standard. While imprint preparations and lysis preparations are quite comparable with respect to distributional features, only one of them suffices to recognize these features, especially peaks at higher ploidies. Using this two-method approach to breast carcinomas, it was possible to distinguish a distribution separate from previously recognized distributions: a distinct triploid peak appears for predominantly high grade malignant ductal carcinomas.
采用乳腺癌病例厚切片的组织学切片、压片标本和酶解法制得的细胞标本，通过图像细胞术研究总核DNA分布。组织学切片是明确识别肿瘤细胞以及用作标准的整个结缔组织细胞的先决条件。虽然压片标本和酶解标本在分布特征方面相当，但只需其中之一即可识别这些特征，尤其是在较高倍体时的峰值。采用这种针对乳腺癌的双方法途径，有可能区分出一种与先前公认的分布不同的分布：在主要为高级别恶性导管癌中出现一个明显的三倍体峰。
