Breter H, Erdmann B
Department of Electron Microscopy, Max Delbrück Center for Molecular Medicine, Berlin-Buch, Germany.
Biotech Histochem. 1993 Jul;68(4):206-10. doi: 10.3109/10520299309104699.
An incubation protocol to immunolabel Lowicryl semithin sections was applied to paraffin probes. To improve the labeling density, colloidal gold complexes of different preparations and sizes were compared. The type of colloidal gold preparation used was found to affect the specificity of the immunostaining. Gold colloid of 5 nm diameter particle size prepared with white phosphorus minimized nonspecific background labeling of beta-casein in paraffin embedded sections of the mammary epithelium of pregnant mice. Gold colloids of 5 nm and 9 nm diameter particle size prepared in varying concentrations of tannic acid generated significant nonspecific staining in similar tissue preparations.
将用于免疫标记Lowicryl半薄切片的孵育方案应用于石蜡探针。为了提高标记密度，对不同制剂和尺寸的胶体金复合物进行了比较。发现所用胶体金制剂的类型会影响免疫染色的特异性。用白磷制备的直径为5 nm的金胶体可使妊娠小鼠乳腺上皮石蜡包埋切片中β-酪蛋白的非特异性背景标记降至最低。在不同浓度的单宁酸中制备的直径为5 nm和9 nm的金胶体在类似的组织制剂中产生了显著的非特异性染色。
