糖尿病易感性BB大鼠的巨噬细胞中氧自由基生成增加。

Oxygen radical production is increased in macrophages from diabetes prone BB rats.

作者信息

Brenner H H, Burkart V, Rothe H, Kolb H

机构信息

Diabetes Research Institute, University of Düsseldorf, Fed. Rep. Germany.

出版信息

Autoimmunity. 1993;15(2):93-8. doi: 10.3109/08916939309043883.

DOI:10.3109/08916939309043883

PMID:8218840

Abstract

Macrophages from autoimmune diabetes prone BB rats were found to produce radical oxygen intermediates (ROI) at an enhanced rate when compared to diabetes resistant BB or normal Wistar rats. The release of ROI was determined by chemiluminescence using in parallel luminol and lucigenin as detector molecules. In diabetes prone BB rats the spontaneous release of ROI was upregulated in macrophages from different compartments, i.e. peritoneum and spleen. Also, maximal output of ROI after activation of macrophages either in vivo by injection of Corynebacterium parvum or in vitro by LPS and IFN was highest for cells from diabetes prone BB rats. This macrophage abnormality was seen in animals prior to recognizable islet inflammation and also was present at the level of macrophages grown in vitro from precursor cells of diabetes prone BB rats. Hypersecretion of oxygen radicals may contribute to Beta cell loss and diabetes development in BB rats.

摘要

与抗糖尿病的BB大鼠或正常的Wistar大鼠相比，发现自身免疫性糖尿病易感BB大鼠的巨噬细胞产生活性氧中间体（ROI）的速率更高。ROI的释放通过化学发光法测定，同时使用鲁米诺和光泽精作为检测分子。在糖尿病易感BB大鼠中，不同部位（即腹膜和脾脏）的巨噬细胞中ROI的自发释放上调。此外，通过注射短小棒状杆菌在体内激活巨噬细胞，或通过脂多糖和干扰素在体外激活巨噬细胞后，糖尿病易感BB大鼠细胞的ROI最大产量最高。这种巨噬细胞异常在可识别的胰岛炎症出现之前的动物中就已出现，并且在从糖尿病易感BB大鼠的前体细胞体外培养的巨噬细胞水平上也存在。氧自由基的过度分泌可能导致BB大鼠的β细胞损失和糖尿病发展。