Dunn M A, Morris A, Jack P L, Hughes M A
Department of Biochemistry and Genetics, Medical School, University of Newcastle upon Tyne, UK.
Plant Mol Biol. 1993 Oct;23(1):221-5. doi: 10.1007/BF00021434.
A cDNA clone (pBLT63) encoding a protein synthesis elongation factor 1 alpha (EF-1 alpha) was isolated from a low-temperature winter barley shoot meristem library by differential screening. The nucleotide sequence of the coding region of the low-temperature-induced barley gene shows very high homology with two EF-1 alpha plant genes from tomato and Arabidopsis. The barley genome contains an EF-1 alpha gene family situated on the short arm of chromosome 2 and the long arm of chromosome 5.
通过差异筛选,从低温处理的冬大麦茎尖分生组织文库中分离出一个编码蛋白质合成延伸因子1α(EF-1α)的cDNA克隆(pBLT63)。低温诱导的大麦基因编码区的核苷酸序列与来自番茄和拟南芥的两个EF-1α植物基因具有很高的同源性。大麦基因组包含一个位于2号染色体短臂和5号染色体长臂上的EF-1α基因家族。