Rathus C, Bower R, Birch R G
Bureau of Sugar Experiment Stations, Brisbane, Australia.
Plant Mol Biol. 1993 Nov;23(3):613-8. doi: 10.1007/BF00019308.
Various chimaeric promoter regions coupled to the uidA beta-glucuronidase gene were evaluated for transient expression strength following electroporation into sugar-cane (monocot) and carrot (dicot) protoplasts. Multiple enhancer elements increased expression in sugar-cane, by up to 400-fold for the artificial Emu promoter relative to the CaMV 35S promoter. The relative expression strengths of promoters varied substantially between the species. Sugar-cane also differed in some respects from previously tested species in the family Poaceae. For example, in sugar-cane the nopaline synthase and CaMV 35S promoters were of equivalent strength, and insertion of Adh1 intron 1 into the 5' transcribed region decreased expression strength.
将与uidAβ-葡萄糖醛酸酶基因偶联的各种嵌合启动子区域导入甘蔗（单子叶植物）和胡萝卜（双子叶植物）原生质体后，通过电穿孔法评估其瞬时表达强度。多个增强子元件可提高甘蔗中的表达，相对于花椰菜花叶病毒35S启动子，人工Emu启动子的表达量提高了400倍。启动子的相对表达强度在不同物种间差异很大。甘蔗在某些方面也与先前测试的禾本科物种不同。例如，在甘蔗中，胭脂碱合酶启动子和花椰菜花叶病毒35S启动子强度相当，而将乙醇脱氢酶1内含子1插入5'转录区域会降低表达强度。
