Horticulture and Crop Science Department, The Ohio State University, The Ohio Agricultural Research and Development Center, 44691, Wooster, OH, USA.
Plant Cell Rep. 1996 Mar;15(7):489-94. doi: 10.1007/BF00232980.
We report a strength comparison of a large variety of monocot and dicot intron-containing fragments inserted in the 5' untranslated leader, between the CaMV 35S promoter and the uidA gene (coding for the ß-glucuronidase: GUS). Relative strengths of the intron-containing fragments were evaluated by comparing transient GUS expression after particle bombardment in embryogenic maize and bluegrass suspension cultures. Our results confirm a dramatic dependence on the presence of an intron for chimeric gene expression in both species. On average, the maize first intron of ubi1 provided the highest enhancement of gene expression in maize and bluegrass (71- and 26-fold enhancement, respectively). Half of the introns tested affected gene expression differently in bluegrass and maize. This suggests that the intron-mediated enhancement of gene expression generally obtained with maize may not be fully applicable to all monocots. We also report enhancement of gene expression (92-fold) in a monocot species by a dicot intron (chsA intron).
我们报告了在玉米胚性悬浮细胞和草地早熟禾悬浮细胞中通过粒子轰击瞬时表达 GUS 后，对 CaMV35S 启动子和 uidA 基因（编码 β-葡萄糖醛酸酶：GUS）之间插入的各种单子叶和双子叶内含子片段的相对强度进行比较。内含子片段的相对强度通过比较来评估。我们的结果证实，在这两个物种中，内含子的存在对嵌合基因表达有显著的依赖性。平均而言，玉米 ubi1 的第一个内含子在玉米和草地早熟禾中的基因表达增强最高（分别为 71 倍和 26 倍）。测试的一半内含子在草地早熟禾和玉米中的基因表达影响不同。这表明，在玉米中获得的内含子介导的基因表达增强可能不完全适用于所有单子叶植物。我们还报告了一个双子叶内含子（chsA 内含子）在单子叶植物中增强基因表达（92 倍）。
