Picton S, Gray J E, Payton S, Barton S L, Lowe A, Grierson D
AFRC Research Group in Plant Gene Regulation, Department of Physiology and Environmental Science, University of Nottingham, Sutton Bonington Campus, Loughborough, UK.
Plant Mol Biol. 1993 Nov;23(3):627-31. doi: 10.1007/BF00019310.
DNA sequencing of a tomato ripening-related cDNA, TOM 92, revealed an open reading frame with homology to several pyridoxal 5'-phosphate histidine decarboxylases, containing the conserved amino acid residues known to bind pyridoxal phosphate and alpha-fluoromethylhistidine, an inhibitor of enzyme activity. TOM 92 mRNA accumulated during early fruit ripening and then declined. Fruit of the ripening-impaired tomato mutant, ripening inhibitor (rin), did not accumulate TOM 92 mRNA, and its accumulation was not restored by treatment of fruit with ethylene. The TOM 92 mRNA was not detected in tomato leaves and unripe fruit.
对一个与番茄成熟相关的cDNA（TOM 92）进行DNA测序，结果显示其开放阅读框与几种磷酸吡哆醛组氨酸脱羧酶具有同源性，含有已知可结合磷酸吡哆醛和α-氟甲基组氨酸（一种酶活性抑制剂）的保守氨基酸残基。TOM 92 mRNA在果实成熟早期积累，随后下降。成熟受损的番茄突变体“成熟抑制剂”（rin）的果实不积累TOM 92 mRNA，用乙烯处理果实也不能恢复其积累。在番茄叶片和未成熟果实中未检测到TOM 92 mRNA。
