Riva S, Villani G, Mastromei G, Mazza G
J Bacteriol. 1976 Aug;127(2):679-90. doi: 10.1128/jb.127.2.679-690.1976.
A Bacillus subtilis temperature-sensitive mutant (PB1653) has been isolated in which the rate of ribonucleic acid (RNA) synthesis sharply decreases after shift to 45 degrees C. Both stable and unstable RNAs are affected by the mutation. The possibility that the block of transcription at high temperature could be due to a "stringent" effect, mediated by an increase in the concentration of "magic spot" nucleotides, has been ruled out. Treatment with chloramphenicol (or streptomycin) rapidly restores the rate of RNA synthesis at 45 degrees C. The synthesis of RNA in the mutant during the early phases of spore germination is not temperature sensitive. The phage-specific transcription during infection with SPP1 phage, at high temperature, is less affected than that of the bacterial chromosome. In vitro experiments indicate that, in the mutant at high temperature, RNA polymerase undergoes a change in template specificity. The rna-53 mutation has been located on the B. subtilis genetic map near the hisA locus.
已分离出一种枯草芽孢杆菌温度敏感突变体（PB1653），在转移至45℃后，其核糖核酸（RNA）合成速率急剧下降。稳定RNA和不稳定RNA均受该突变影响。高温下转录受阻可能是由“魔斑”核苷酸浓度增加介导的“严谨”效应所致，这种可能性已被排除。用氯霉素（或链霉素）处理可迅速恢复45℃时的RNA合成速率。在孢子萌发早期，突变体中的RNA合成对温度不敏感。在高温下感染SPP1噬菌体期间，噬菌体特异性转录比细菌染色体的转录受影响小。体外实验表明，在高温下的突变体中，RNA聚合酶的模板特异性发生了变化。rna - 53突变已定位在枯草芽孢杆菌遗传图谱上靠近hisA基因座的位置。
