Milanesi G, Brevet J
Nucleic Acids Res. 1974 Mar;1(3):397-412. doi: 10.1093/nar/1.3.397.
Partially purified extracts from sporulating Bacillussubtilis cultures transcribed different natural DNAs with different efficiencies. This template specificity results in an increased or a decreased synthetic activity with respect to extracts from vegetative cells, depending on the template used. With SPP1 DNA a decrease in activity occurs, whereas with T7 DNA an increased activity was observed, which is due to a higher efficiency of initiation. This is not an intrinsic property of RNA polymerase, but is due to some fraction(s) which can be separated from the enzyme. Together with invivo experiments on transcription and SPP1 phage production during sporulation, these results suggest a possible role of promoter recognition in sporulation.
来自枯草芽孢杆菌芽孢形成培养物的部分纯化提取物以不同效率转录不同的天然DNA。这种模板特异性导致相对于营养细胞提取物的合成活性增加或降低，这取决于所使用的模板。对于SPP1 DNA，活性降低，而对于T7 DNA，观察到活性增加，这是由于起始效率更高。这不是RNA聚合酶的固有特性，而是由于可以与该酶分离的某些组分。连同关于芽孢形成期间转录和SPP1噬菌体产生的体内实验，这些结果表明启动子识别在芽孢形成中可能起作用。
