Goguel A F, Crainic K, Ducailar A, Ouin M
Etalonorme, University Paris V, Hematology, Ambroise Paré Hospital, Boulogne, France.
Biol Cell. 1993;78(1-2):79-84. doi: 10.1016/0248-4900(93)90118-x.
A 3-year interlaboratory proficiency testing for lymphocyte subset phenotyping was initiated as part of the Etalonorme national quality control program. Specimens consisted of fresh whole blood and of lyophilised mononuclear cells (Cytotrol Coulter). The number of participating laboratories was 62 in 1990, 99 in 1991 and 129 in 1992. Statistical analysis indicated that results of phenotyping, expressed as percentages of positive cells, are not related to reagents, instruments or differences in methodology (like, eg time and temperature of incubation). The highest dispersion was observed for total lymphocyte counts and was found to correlate with the type of calibration of the instrument. The coefficients of variation for different lymphocyte subsets were similar if phenotyping was performed on whole blood or lyophilised cells and varied inversely with the percentage of positive cells in each specimen. The consistency of the results indicated that they could serve as a basis for clinical decisions.
作为Etalonorme国家质量控制计划的一部分，启动了一项为期3年的淋巴细胞亚群表型分析实验室间能力验证。样本包括新鲜全血和冻干单核细胞（Cytotrol Coulter）。1990年有62个参与实验室，1991年有99个，1992年有129个。统计分析表明，以阳性细胞百分比表示的表型分析结果与试剂、仪器或方法学差异（如孵育时间和温度）无关。总淋巴细胞计数的离散度最高，且发现与仪器校准类型相关。如果对全血或冻干细胞进行表型分析，不同淋巴细胞亚群的变异系数相似，且与每个样本中阳性细胞的百分比成反比。结果的一致性表明，它们可为临床决策提供依据。
