Van Blerk Marian, Bernier Michel, Bossuyt Xavier, Chatelain Bernard, D'Hautcourt Jean-Luc, Demanet Christian, Kestens Luc, Van Bockstaele Dirk, Crucitti Tania, Libeer Jean-Claude
Department of Clinical Biology, Scientific Institute of Public Health, Brussels, Belgium.
Clin Chem Lab Med. 2003 Mar;41(3):323-30. doi: 10.1515/CCLM.2003.052.
In 2000, the Belgian Scientific Institute of Public Health introduced a voluntary external quality assessment scheme for lymphocyte immunophenotyping. This paper provides an analysis of the first six surveys. Specimens consisted of fresh EDTA-anticoagulated whole blood and were sent by overnight mail. The 41 participants were surveyed for methodology and were asked to report white blood cell count, percentage of lymphocytes, and percentages and absolute numbers of CD3+, CD4+, CD8+, and CD19+ cells. Median intralaboratory coefficients of variation were 1.0, 1.3, 1.7, and 3.2% for CD3+, CD4+, CD8+, and CD19+ cell percentages, respectively. Interlaboratory variability was consistently lower than 6.5% for CD3+ and CD4+CD3+ cell percentages, and lower than 9.5% for CD8+CD3+ cell percentages. Median coefficients of variation for the absolute values were higher, ranging from 10.1% for CD4+CD3+ cells to 16.5% for CD19+ cells. The percentage of CD4+CD3+ and CD8+ CD3+ cells was in several samples significantly lower than the percentage of total CD4+ and CD8+ cells. The number of laboratories measuring total CD4+ and CD8+ cells decreased by 30% during the programme. Between-laboratory variability remained stable over time. Analysis of individual laboratory performance indicated that some laboratories markedly improved their results.
2000年，比利时公共卫生科学研究所推出了一项淋巴细胞免疫分型的自愿外部质量评估计划。本文对前六项调查进行了分析。样本为新鲜的乙二胺四乙酸（EDTA）抗凝全血，通过隔夜快递寄送。对41名参与者进行了方法学调查，并要求他们报告白细胞计数、淋巴细胞百分比以及CD3 +、CD4 +、CD8 +和CD19 +细胞的百分比及绝对数量。CD3 +、CD4 +、CD8 +和CD19 +细胞百分比的实验室内部变异系数中位数分别为1.0%、1.3%、1.7%和3.2%。CD3 +和CD4 + CD3 +细胞百分比的实验室间变异始终低于6.5%，CD8 + CD3 +细胞百分比的实验室间变异低于9.5%。绝对值的变异系数中位数较高，从CD4 + CD3 +细胞的10.1%到CD19 +细胞的16.5%不等。在几个样本中，CD4 + CD3 +和CD8 + CD3 +细胞的百分比明显低于总CD4 +和CD8 +细胞的百分比。在该计划期间，测量总CD4 +和CD8 +细胞的实验室数量减少了30%。实验室间变异随时间保持稳定。对单个实验室表现的分析表明，一些实验室显著改善了其结果。
