Jayat C, Ratinaud M H
Service Universitaire de Cytométrie, Faculté des Sciences, Limoges, France.
Biol Cell. 1993;78(1-2):15-25. doi: 10.1016/0248-4900(93)90110-z.
Numerous flow cytometric analyses are based on DNA content studies. We have considered firstly monoparametric cell cycle analyses, which only take DNA content into account, but are sometimes of limited interest. Then, we have presented multiparametric analyses, which can be used to improve cycle phase identification by taking simultaneously into account DNA and other cellular components, or by considering some events occurring during cell cycle. Finally, we have discussed monoparametric and multiparametric cell cycle analysis interest in various application fields, particularly in pharmacology, toxicology, tumoral pathology and higher plant system studies.
众多的流式细胞术分析是基于DNA含量研究的。我们首先考虑了单参数细胞周期分析，这种分析仅考虑DNA含量，但有时其价值有限。然后，我们介绍了多参数分析，它可通过同时考虑DNA和其他细胞成分，或通过考虑细胞周期中发生的一些事件来用于改进细胞周期阶段的识别。最后，我们讨论了单参数和多参数细胞周期分析在各种应用领域的价值，特别是在药理学、毒理学、肿瘤病理学和高等植物系统研究中的价值。
