Thoma S, Kaneko Y, Somerville C
MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing 48824.
Plant J. 1993 Mar;3(3):427-36. doi: 10.1046/j.1365-313x.1993.t01-25-00999.x.
Lipid transfer proteins (LTPs), mediate the transfer of phospholipids between membranes in vitro. However, the in vivo function of LTPs is not known. To determine the precise location of a non-specific LTP from Arabidopsis, a cDNA clone was used to produce an Arabidopsis LTP:protein A fusion. Antibodies raised against the fusion were used to localize the Arabidopsis LTP by immunoelectron microscopy. LTP was found to be located in the cell wall, mainly in epidermal cells. This location appears to be inconsistent with the proposed role of the protein in intracellular lipid transfer.
脂质转移蛋白（LTPs）在体外介导磷脂在膜之间的转移。然而，LTPs在体内的功能尚不清楚。为了确定拟南芥中非特异性LTP的确切位置，使用一个cDNA克隆来产生拟南芥LTP与蛋白A的融合体。针对该融合体产生的抗体用于通过免疫电子显微镜对拟南芥LTP进行定位。发现LTP位于细胞壁中，主要在表皮细胞中。这一位置似乎与该蛋白在细胞内脂质转移中所提出的作用不一致。
