Yang W C, Katinakis P, Hendriks P, Smolders A, de Vries F, Spee J, van Kammen A, Bisseling T, Franssen H
Department of Molecular Biology, Agricultural University, Wageningen, The Netherlands.
Plant J. 1993 Apr;3(4):573-85. doi: 10.1046/j.1365-313x.1993.03040573.x.
In this paper, the soybean 'early nodulin' clone pGmENOD40 is characterized. The GmENOD40 encoded protein does not contain methionine and does not show homology to proteins identified so far. In situ hybridizations showed that this gene has a complex expression pattern during development of determinate soybean nodules. At early stages of development transcription is induced in dividing root cortical cells, the nodule primordium and the pericycle of the root vascular bundle. In mature soybean nodules, the gene is expressed in the uninfected cells of the central tissue and in the pericycle of the nodule vascular bundles. Studies on nodules devoid of intracellular bacteria and infection threads, showed that the expression of the gene in the nodule primordium is induced in these empty nodules, while the induction of the GmENOD40 gene in the nodule vascular bundle requires the presence of intracellular bacteria or infection threads. A pea cDNA clone homologous to GmENOD40 was isolated to enable in situ hybridization studies on indeterminate nodules. The expression patterns in both determinate and indeterminate nodules suggests that the ENOD40 protein might have a transport function.
本文对大豆“早期结瘤素”克隆体pGmENOD40进行了表征。GmENOD40编码的蛋白质不含甲硫氨酸，且与迄今鉴定出的蛋白质无同源性。原位杂交显示，该基因在有限生长型大豆根瘤发育过程中具有复杂的表达模式。在发育早期，转录在分裂的根皮层细胞、根瘤原基和根维管束的中柱鞘中被诱导。在成熟的大豆根瘤中，该基因在中央组织的未感染细胞和根瘤维管束的中柱鞘中表达。对不含细胞内细菌和侵染线的根瘤进行的研究表明，该基因在根瘤原基中的表达在这些空根瘤中被诱导，而GmENOD40基因在根瘤维管束中的诱导需要细胞内细菌或侵染线的存在。分离出一个与GmENOD40同源的豌豆cDNA克隆体，以便对无限生长型根瘤进行原位杂交研究。有限生长型和无限生长型根瘤中的表达模式表明，ENOD40蛋白可能具有转运功能。
