Portas M R, Hogan N A, Hill H R
J Lab Clin Med. 1976 Aug;88(2):339-44.
Clinical isolates of group D streptococci have been examined by counterimmunoelectrophoresis (CIE) to determine if this method might be of value in the rapid identification of the members of this group. Group D organisms identified by classical biochemical and serologic procedures were inoculated into Todd Hewitt broth and incubated at 37 degrees C. After 4 hours of incubation, free group D streptococcal antigen was detected in broth cultures from 70 of 70 group D enterococcal strains. Precipitin lines were readily visible immediately after electrophoresis in each case. In contrast, 10 nonenterococcal group D strains failed to produce detectable free group D antigen in the Todd Hewitt broth after 4, 24, or 48 hours incubation. Such antigen was detectable, however, after 48 hours incubation in Mueller Hinton broth containing 10 grams of glucose per liter. Representative strains of group A, C, G, and B streptococi, Streptococcus viridans and Streptococcus pneumoniae grown in Todd-Hewitt broth for 4 hours gave no precipitin lines when reacted against adsorbed monospecific group D antiserum. Group D antigen was also detected in broth cultures of direct swabs and in 18-hour blood culture broths. CIE appears to be a rapid and sensitive procedure for identification of the enterococcal strains of group D streptococci from clinical isolates.
已通过对流免疫电泳（CIE）对D群链球菌的临床分离株进行检测，以确定该方法在快速鉴定该菌群成员方面是否有价值。将通过经典生化和血清学方法鉴定的D群微生物接种到托德-休伊特肉汤中，于37℃培养。培养4小时后，在70株D群肠球菌菌株的肉汤培养物中检测到游离的D群链球菌抗原。每次电泳后立即清晰可见沉淀线。相比之下，10株非肠球菌D群菌株在培养4、24或48小时后，在托德-休伊特肉汤中未能产生可检测到的游离D群抗原。然而，在每升含10克葡萄糖的米勒-欣顿肉汤中培养48小时后可检测到此类抗原。在托德-休伊特肉汤中培养4小时的A、C、G和B群链球菌、草绿色链球菌和肺炎链球菌的代表性菌株，与吸附的单特异性D群抗血清反应时未出现沉淀线。在直接拭子的肉汤培养物和18小时血培养肉汤中也检测到了D群抗原。对流免疫电泳似乎是一种从临床分离株中快速、灵敏地鉴定D群链球菌肠球菌菌株的方法。
