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小鼠胸腺细胞上的Ly-3抗原：免疫沉淀及分子量特性分析

The Ly-3 antigens on mouse thymocytes: immune precipitation and molecular weight characterization.

作者信息

Durda P J, Gottlieb P D

出版信息

J Exp Med. 1976 Aug 1;144(2):476-93. doi: 10.1084/jem.144.2.476.

DOI:10.1084/jem.144.2.476
PMID:822115
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2190382/
Abstract

Specific anti-Ly sera were employed to precipitate Ly antigens from Nonidet P-40 extracts of mouse thymocytes labeled with 125I using lactoperoxidase and with NaB3H4 using galactose oxidase. Thymocytes from mice of the congenic strains C57BL/6J (Ly-2.2, Ly-3.2 positive), C57BL/6Ly-2a, Ly-3a (Ly-2.1, Ly-3.1 positive) and C57BL/6-Ly-2a (Ly-2.1, Ly-3.2-positive) were used as sources of labeled antigens and as immune adsorbants to permit evaluation of the specificity of each anti-Ly serum employed. Results of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) under reducing conditions are consistent with the Ly-3.1 antigen containing a glycoprotein subunit with an apparent mol wt of 35,000 daltons. Specific precipitates obtained using anti-Ly-2.1 serum yielded SDS-PAGE profiles identical to that obtained with anti-Ly-3.1 serum, suggesting that the Ly-2 and Ly-3 antigens have the same molecular weight distribution. The relationships of these results to the observed close genetic and topological linkage of Ly-2 and Ly-3 and to the genetic linkage of these loci with the IB-peptide marker, a mouse Bk-region polymorphism, are discussed.

摘要

使用特异性抗Ly血清从用乳过氧化物酶标记125I和用半乳糖氧化酶标记NaB3H4的小鼠胸腺细胞的Nonidet P - 40提取物中沉淀Ly抗原。同基因品系C57BL/6J（Ly - 2.2、Ly - 3.2阳性）、C57BL/6Ly - 2a、Ly - 3a（Ly - 2.1、Ly - 3.1阳性）和C57BL/6 - Ly - 2a（Ly - 2.1、Ly - 3.2阳性）小鼠的胸腺细胞用作标记抗原的来源和免疫吸附剂，以评估所使用的每种抗Ly血清的特异性。在还原条件下进行的十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳（SDS - PAGE）结果与Ly - 3.1抗原含有一个表观分子量为35,000道尔顿的糖蛋白亚基一致。使用抗Ly - 2.1血清获得的特异性沉淀物产生的SDS - PAGE图谱与使用抗Ly - 3.1血清获得的图谱相同，表明Ly - 2和Ly - 3抗原具有相同的分子量分布。讨论了这些结果与观察到的Ly - 2和Ly - 3紧密遗传和拓扑连锁以及这些基因座与IB - 肽标记（一种小鼠Bk区域多态性）的遗传连锁之间的关系。

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引用本文的文献

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Immunogenetics. 1980;10(6):545-55. doi: 10.1007/BF01572589.
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Lyt-2-/Lyt 3- variants of a cloned cytolytic T cell line lack an antigen receptor functional in cytolysis.一个克隆的细胞溶解T细胞系的Lyt-2-/Lyt 3-变体缺乏在细胞溶解中起作用的抗原受体。
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Cell surface immunoglobulin. IV. Distribution among thymocytes, bone mrrow cells, and their derived populations.细胞表面免疫球蛋白。IV. 在胸腺细胞、骨髓细胞及其衍生群体中的分布。
J Exp Med. 1972 Jul 1;136(1):81-93. doi: 10.1084/jem.136.1.81.

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