Ebeling H
J Clin Chem Clin Biochem. 1976 May;14(5):205-11.
A method is described for the quantitative determination of fibrinogen equivalents in citrated blood before and after removal of the fibrinogen by coagulation. The method employs an immunological reaction, which is measured by mechanized nephelometry. Thus the sum of all the fibrinogen equivalents can be compared with the concentration of "cleavage products". The difference between the two readings represents the concentration of coagulable fibrinogen. For the fibrinogen concentration of 11.15 mg/l, variation coefficients of 2.81% in series and 3.31% for day to day were obtained. The accuracy of a new method was tested under conditions of fibrinogenolysis, and in the presence of heparin, by comparison with simple radial immunodiffusion, and with the "coagulation physiological rapid method for the determination of fibrinogen" after Clauss ((1957), Acta-Haematol, 17, 237-246). In addition 180 citrated blood samples of different patients were compared with the simple radial immunodiffusion, the method of Clauss and the new method. Only the new rapid method appears to measure plausible fibrinogen values, and permits a clear differentiation between coagulable and non-coagulable fibrinogen equivalents. It can be recommended for clinical practice.
本文描述了一种用于定量测定枸橼酸盐血液中纤维蛋白原当量的方法，该方法在通过凝血去除纤维蛋白原之前和之后进行。该方法采用免疫反应，通过机械比浊法进行测量。因此，所有纤维蛋白原当量的总和可以与“裂解产物”的浓度进行比较。两次读数之间的差异代表可凝固纤维蛋白原的浓度。对于纤维蛋白原浓度为11.15mg/l的情况，系列变异系数为2.81%，每日变异系数为3.31%。通过与简单的放射免疫扩散法以及与Clauss（(1957)，《血液学学报》，17，237 - 246）之后的“凝血生理快速纤维蛋白原测定法”进行比较，在纤维蛋白溶解条件下以及肝素存在的情况下测试了一种新方法的准确性。此外，将180份不同患者的枸橼酸盐血液样本与简单放射免疫扩散法、Clauss法和新方法进行了比较。只有新的快速方法似乎能测量出合理的纤维蛋白原值，并能明确区分可凝固和不可凝固的纤维蛋白原当量。它可推荐用于临床实践。
