Basu J, Duttagupta C, Vermund S H, Ahn C, Palan P R, Romney S L
Department of Obstetrics and Gynecology, Albert Einstein College of Medicine, Bronx, New York 10461.
Cancer Invest. 1993;11(6):652-9. doi: 10.3109/07357909309046937.
The study was designed to test the hypothesis whether cervical dysplasias of the more severe grades are associated with elevated erythrocyte glutathione levels. Subjects were women who obtained Pap tests and were subsequently found (1) not to have any cervical lesions or (2) to have colposcopically visualized, biopsy-confirmed cervical abnormalities histopathologically diagnosed as mild, moderate, severe dysplasias, or carcinoma in situ (CIS). The erythrocyte levels of reduced glutathione (GSH), oxidized glutathione (GSSG), glutathione reductase (GR), glucose-6-phosphate dehydrogenase (G6PD), and 6-phosphogluconate dehydrogenase (6PGD) were analyzed from coded peripheral venous blood samples. GSH and GR concentrations increased with increasing severity of dysplasia. Exploratory data analysis and multiple pairwise comparisons suggested comparable levels of the glutathione-related variables between these histopathological pairs: (1) mild and moderate dysplasias or (2) severe dysplasia and CIS. We suggest that the changes in erythrocyte glutathione-related indices in conjunction with histopathological diagnosis may have the potential to distinguish between low- and high-grade cervical dysplastic lesions.
本研究旨在检验更严重级别的宫颈发育异常是否与红细胞谷胱甘肽水平升高相关这一假设。研究对象为接受巴氏试验的女性，随后发现她们（1）没有任何宫颈病变，或（2）有经阴道镜观察、活检证实的宫颈异常，组织病理学诊断为轻度、中度、重度发育异常或原位癌（CIS）。从编码的外周静脉血样本中分析还原型谷胱甘肽（GSH）、氧化型谷胱甘肽（GSSG）、谷胱甘肽还原酶（GR）、葡萄糖-6-磷酸脱氢酶（G6PD）和6-磷酸葡萄糖酸脱氢酶（6PGD）的红细胞水平。GSH和GR浓度随着发育异常严重程度的增加而升高。探索性数据分析和多重成对比较表明，这些组织病理学配对之间的谷胱甘肽相关变量水平相当：（1）轻度和中度发育异常，或（2）重度发育异常和原位癌。我们认为，红细胞谷胱甘肽相关指标的变化结合组织病理学诊断可能有潜力区分低级别和高级别宫颈发育异常病变。
