原位杂交：一种检测慢性淋巴细胞白血病中12号染色体三体的简单且灵敏的方法。

In situ hybridization: a simple and sensitive method for detection of trisomy 12 in chronic lymphocytic leukemia.

作者信息

Lalkin A, Lishner M, Gaber E, Manor Y, Fejgin M, Ravid M, Amiel A

机构信息

Department of Medicine, Meir Hospital, Kfar Saba, Israel.

出版信息

Cancer Genet Cytogenet. 1993 Oct 1;70(1):21-4. doi: 10.1016/0165-4608(93)90126-7.

DOI:10.1016/0165-4608(93)90126-7

PMID:8221608

Abstract

Chromosome aberrations are detected in only 50% of patients with chronic lymphocytic leukemia (CLL), owing usually to the low mitotic rate exhibited by the neoplastic lymphocytes. Fluorescence in situ hybridization (FISH) is a simple method for identifying numerical abnormalities of the target chromosome in interphase nuclei. Therefore, we used the FISH procedure with chromosome 12-specific a-satellite probe to evaluate 19 patients with CLL. Trisomy 12 was detected in interphase cells of 12 patients (63%). Cytogenetic analysis, performed in nine patients, yielded trisomy 12 in four (44%). FISH detected three patients with trisomy 12 in whom conventional cytogenetic method yielded a normal karyotype. FISH is a simple, reliable, and sensitive method for detection of trisomy 12 in patients with CLL.

摘要

仅50%的慢性淋巴细胞白血病（CLL）患者可检测到染色体畸变，这通常是由于肿瘤淋巴细胞的有丝分裂率较低。荧光原位杂交（FISH）是一种用于识别间期细胞核中目标染色体数目异常的简单方法。因此，我们使用针对12号染色体的α卫星探针的FISH程序来评估19例CLL患者。在12例患者（63%）的间期细胞中检测到12号染色体三体。对9例患者进行的细胞遗传学分析显示，其中4例（44%）存在12号染色体三体。FISH检测到3例12号染色体三体患者，而传统细胞遗传学方法显示其核型正常。FISH是一种用于检测CLL患者12号染色体三体的简单、可靠且灵敏的方法。