Liu Y Q, Gao J
Department of Pathology, Chinese Academy of Medical Sciences, Beijing.
Zhonghua Bing Li Xue Za Zhi. 1993 Jun;22(3):150-3.
The in vitro invasiveness of LA1, LAD, LA5 variants of mouse lung adenocarcinoma (LA795) with different metastatic potentials were examined. Precultured heart fragment (PHF) was used as the target organ, and spheroid aggregates of LA1, LAD, LA5 cells were prepared by cultivation of cell suspension on a gyratory shaker. The invasion-complex was formed by confronting of the PHF and cell aggregate. The invasion-complex was then examined at different time intervals histologically and morphometrically. The micro-computer-aided image analysis system was employed to measure the total area (TA) of the invasion complex and the tumor tissue area (subarea, SA). The ratio of SA/TA was used in estimating the invasion ability. It was shown that the invasion of LA1, LAD and LA5 was progressive in nature and the invasive capability of these three variants were correlative with their metastatic potentials.
对具有不同转移潜能的小鼠肺腺癌（LA795）的LA1、LAD、LA5变体的体外侵袭性进行了检测。将预培养的心脏片段（PHF）用作靶器官，通过在回转振荡器上培养细胞悬液制备LA1、LAD、LA5细胞的球体聚集体。通过将PHF与细胞聚集体相对放置形成侵袭复合体。然后在不同时间间隔对侵袭复合体进行组织学和形态计量学检查。采用微计算机辅助图像分析系统测量侵袭复合体的总面积（TA）和肿瘤组织面积（子区域，SA）。SA/TA比值用于评估侵袭能力。结果表明，LA1、LAD和LA5的侵袭具有渐进性，这三种变体的侵袭能力与其转移潜能相关。
