Shimojo N, Naka K, Uenoyama H, Hamamoto K, Yoshioka K, Okuda K
Department of Laboratory Medicine, Osaka City University Medical School, Japan.
Clin Chem. 1993 Nov;39(11 Pt 1):2312-4.
We have developed an assay system for measuring lactate in whole blood, consisting of a single-use strip of an enzyme-coated electrode and a small meter. The electrode strip is made of three plastic films: a cover sheet, a spacer, and an insulation layer printed with electrodes that are coated with lactate oxidase (EC 1.1.3.x) and ferricyanide as an electron mediator. The meter measures the magnitude of the anodic current of the reduced mediator by the enzymatic reaction and displays the lactate concentration 60 s after a blood sample (5 microL) is applied. The calibration curve was linear up to 20 mmol/L, and the between-run CVs at three concentrations were 1.7-8.4%. Lactate concentrations determined by this method (y) in blood samples from healthy individuals before and after exercise agreed with the results obtained by the conventional enzymatic method (x): y = 0.97x - 0.3, Sy/x = 0.7. This assay provides a rapid and convenient test for measuring blood lactate concentrations.
我们开发了一种用于测量全血中乳酸的检测系统，该系统由一条一次性使用的酶涂层电极试纸条和一个小型仪器组成。电极试纸条由三层塑料薄膜制成：一个覆盖片、一个垫片和一个印有电极的绝缘层，电极上涂有乳酸氧化酶（EC 1.1.3.x）和作为电子媒介体的铁氰化物。该仪器通过酶促反应测量还原媒介体的阳极电流大小，并在加入血样（5微升）60秒后显示乳酸浓度。校准曲线在20 mmol/L范围内呈线性，三种浓度下的批间变异系数为1.7 - 8.4%。通过该方法（y）测定的健康个体运动前后血样中的乳酸浓度与传统酶法（x）所得结果一致：y = 0.97x - 0.3，Sy/x = 0.7。该检测方法为测量血乳酸浓度提供了一种快速便捷的检测手段。
