Pfeiffer D, Möller B, Klimes N, Szeponik J, Fischer S
BST Bio Sensor Technologie GmbH, Berlin, Germany.
Biosens Bioelectron. 1997;12(6):539-50. doi: 10.1016/s0956-5663(97)00014-6.
An amperometric lactate oxidase catheter has been developed for in vivo application to real-time lactate monitoring. The electrochemical behaviour of the 1 x 3 mm Pt-Ag/AgCl thin film electrode is not significantly influenced by lactate oxidase-polyurethane covering. Gamma-irradiation (25 kGy) is suitable for the sterilization procedure. The final lactate catheter is characterized by a linear concentration range between 0.5 and 20 mmol/l lactate with a sensitivity around 2 nA mmol-1 l-1 lactate. The accuracy is demonstrated by the measurement of control sera. Both physiological and pathological materials correlate well with the declared values. The dry stored lactate catheter needs about 10 min for hydration and is characterized by response times t98% of less than 2 min. Ex vivo whole blood measurements using the lactate catheter (y) give a correlation with the BIOSEN Med L (x) of y = (1.010x + 0.513) mmol/l (r = 0.9748). Lactate values obtained by continuous catheter operation ex vivo correlate well with those obtained by BIOSEN Med L. First subcutaneous implantation (dog) underlines the characteristics obtained ex vivo: after 30 min hydration the lactate catheter follows the lactate concentration measured ex vivo with samples from the leg vein by BIOSEN Med L.
一种用于体内实时乳酸监测的安培型乳酸氧化酶导管已被开发出来。1×3毫米的铂-氯化银薄膜电极的电化学行为受乳酸氧化酶-聚氨酯覆盖层的影响不显著。γ射线辐照（25千戈瑞）适用于灭菌程序。最终的乳酸导管的特点是乳酸浓度线性范围在0.5至20毫摩尔/升之间，灵敏度约为2纳安·毫摩尔⁻¹·升⁻¹乳酸。通过对照血清的测量证明了其准确性。生理和病理材料与公布的值相关性良好。干燥保存的乳酸导管水化需要约10分钟，其响应时间t98%小于2分钟。使用乳酸导管（y）进行的离体全血测量与BIOSEN Med L（x）的相关性为y =（1.010x + 0.513）毫摩尔/升（r = 0.9748）。通过导管在离体条件下连续操作获得的乳酸值与通过BIOSEN Med L获得的值相关性良好。首次皮下植入（狗）突出了在离体条件下获得的特性：水化30分钟后，乳酸导管跟踪通过BIOSEN Med L从腿部静脉采集的样本在离体条件下测量的乳酸浓度。
