一种基于凝集素的新型酶联免疫吸附测定法，用于检测血清中的IgA1和分泌物中的分泌型IgA1。
A novel lectin-based enzyme-linked immunosorbent assay for the measurement of IgA1 in serum and secretory IgA1 in secretions.
作者信息
Cullina M J, Greally J F
机构信息
Immunology Department, University College Hospital, Galway, Ireland.
出版信息
Clin Chim Acta. 1993 Jul 16;216(1-2):23-38. doi: 10.1016/0009-8981(93)90136-r.
Jackfruit lectin (Jacalin) specifically binds IgA1 and secretory IgA1 (sIgA1). Jacalin was successfully purified, and used as the immobilized phase in novel noncompetitive enzyme-linked immunosorbent assays (ELISAs) for the measurement of IgA1 in serum and sIgA1 in saliva, using jacalin affinity purified material as standards. A non-competitive ELISA for the measurement of total sIgA was also developed using commercially available reagents.
波罗蜜凝集素（jacalin）能特异性结合IgA1和分泌型IgA1（sIgA1）。已成功纯化出jacalin，并将其用作新型非竞争性酶联免疫吸附测定（ELISA）中的固定相，用于检测血清中的IgA1和唾液中的sIgA1，以jacalin亲和纯化材料作为标准品。还使用市售试剂开发了一种用于检测总sIgA的非竞争性ELISA。
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