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Creating a conditional mutation of Wnt-1 by antisense transgenesis provides evidence that Wnt-1 is not essential for spermatogenesis.

作者信息

Erickson R P, Lai L W, Grimes J

机构信息

Steele Memorial Children's Research Center, University of Arizona, College of Medicine, Tucson 85724.

出版信息

Dev Genet. 1993;14(4):274-81. doi: 10.1002/dvg.1020140405.

DOI:10.1002/dvg.1020140405
PMID:8222343
Abstract

We have used mice transgenic for an antisense construct for Wnt-1 to study the role of this gene in post-meiotic sperm development. The human PGK-2 promoter provided levels of Wnt-1 antisense mRNA in testes in 5 transgenic lines greatly in excess of Wnt-1 mRNA concentrations, and Wnt-1 mRNA levels were greatly decreased in the lines, by 98% in three of them. There was a general correlation between copy number of the insert, levels of antisense RNA, and decreases in mRNA. There was little effect of the antisense transgene on fertility or testicular histology suggesting that normal levels of Wnt-1 transcript are not essential for spermatogenesis.

摘要

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