Kurata S, Matsumoto M, Hoshi M, Nakajima H
Department of Biochemical Genetics, Tokyo Medical and Dental University, Japan.
Eur J Biochem. 1993 Oct 15;217(2):633-8. doi: 10.1111/j.1432-1033.1993.tb18286.x.
The activation of the heme oxygenase (HO) gene during development of mouse male germ cells was examined by nuclear run-off assay. Results showed that the HO gene was activated in male germ cells, especially in spermatogonia and that the mRNA was stored throughout meiosis. In contrast, Western blotting analysis revealed that HO was expressed during spermatogenesis to spermatocytes. As the mouse HO gene is known to have a 12-o-tetradecanoyl-phorbol 13-acetate(TPA)-responsive element (TRE) in its upstream region and is activated by TPA in mouse M1 cells, we also examined the activation of the nuclear proto-oncogenes fos and jun, which are activators of TRE. Both genes were found to be activated with expression of their protein products only in spermatogonia. Moreover activation of fos, jun and HO genes in spermatogonia was strongly inhibited by a c-kinase inhibitor. These results suggest that the HO gene is activated at the spermatogonia stage by a fos/jun heterodimer complex (AP1) through c-kinase activation.
通过核转录分析检测了小鼠雄性生殖细胞发育过程中血红素加氧酶（HO）基因的激活情况。结果显示，HO基因在雄性生殖细胞中被激活，尤其是在精原细胞中，并且其mRNA在整个减数分裂过程中都有储存。相比之下，蛋白质印迹分析表明，HO在精子发生至精母细胞阶段表达。由于已知小鼠HO基因在其上游区域有一个12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯（TPA）反应元件（TRE），并且在小鼠M1细胞中被TPA激活，我们还检测了核原癌基因fos和jun的激活情况，它们是TRE的激活剂。结果发现，只有在精原细胞中这两个基因才会随着其蛋白质产物的表达而被激活。此外，精原细胞中fos、jun和HO基因的激活受到c - 激酶抑制剂的强烈抑制。这些结果表明，HO基因在精原细胞阶段通过fos/jun异源二聚体复合物（AP1）经c - 激酶激活而被激活。
