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用一种简单方法测定紧密上皮的基本电特性。

Basic electrical properties of tight epithelia determined with a simple method.

作者信息

Erlij D

出版信息

Pflugers Arch. 1976 Jun 29;364(1):91-3. doi: 10.1007/BF01062917.

DOI:10.1007/BF01062917
PMID:822395
Abstract

A simple method for the routine mounting of epithelia which greatly reduces or eliminates faulty sealing at the edge is described. The values of potential and resistance observed in the frog skin and toad urinary bladder are comparable to those found with more complicated methods designed to minimize edge damage. In agreement with observations in other epithelia, a direct relationship between short circuit and conductance is observed for frog skin and toad urinary bladder, implying that the relationship is a general feature of tight epithelia. Furthermore, the high values of transepithelial resistance imply that the lower limit for the paracellular shunt pathway resistance should be reset upwards.

摘要

本文描述了一种用于常规上皮组织固定的简单方法,该方法可极大减少或消除边缘处的密封故障。在蛙皮和蟾蜍膀胱中观察到的电位和电阻值,与使用旨在最小化边缘损伤的更复杂方法所获得的值相当。与在其他上皮组织中的观察结果一致,在蛙皮和蟾蜍膀胱中观察到短路与电导之间存在直接关系,这意味着这种关系是紧密上皮组织的一个普遍特征。此外,跨上皮电阻的高值意味着细胞旁分流途径电阻的下限应向上重新设定。

相似文献

1
Basic electrical properties of tight epithelia determined with a simple method.用一种简单方法测定紧密上皮的基本电特性。
Pflugers Arch. 1976 Jun 29;364(1):91-3. doi: 10.1007/BF01062917.
2
Blockage of Na+ currents through poorly selective cation channels in the apical membrane of frog skin and toad urinary bladder.蛙皮和蟾蜍膀胱顶端膜中选择性差的阳离子通道对钠离子电流的阻断作用。
Pflugers Arch. 1991 Apr;418(3):193-203. doi: 10.1007/BF00370514.
3
Effect of amiloride on conductance of toad urinary bladder.氨氯吡咪对蟾蜍膀胱电导率的影响。
J Membr Biol. 1980 Jan 31;52(1):61-7. doi: 10.1007/BF01869006.
4
Microelectrode study of K+ accumulation by tight epithelia: I. Baseline values of split frog skin and toad urinary bladder.紧密上皮细胞对钾离子蓄积的微电极研究:I. 离体青蛙皮肤和蟾蜍膀胱的基线值
J Membr Biol. 1983;72(3):183-93. doi: 10.1007/BF01870585.
5
Current-voltage relations of the basolateral membrane in tight amphibian epithelia: use of nystatin to depolarize the apical membrane.紧密两栖类上皮细胞基底外侧膜的电流-电压关系:使用制霉菌素使顶端膜去极化。
J Membr Biol. 1984;77(3):213-22. doi: 10.1007/BF01870570.
6
Exocytotic events unrelated to regulation of water permeability in amphibian tight epithelia: effects of oxytocin, PMA and insulin on membrane capacitance, water and Na+ transport.两栖类致密上皮中与水通透性调节无关的胞吐事件:催产素、佛波酯和胰岛素对膜电容、水和钠离子转运的影响
Biol Cell. 1989;66(1-2):53-8.
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Electrical properties of amphibian urinary bladder epithelia. I. Inverse relationship between potential difference and resistance in tightly mounted preparations.
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8
Transepithelial current-voltage relationships of toad urinary bladder and colon. Estimates of ENaA and shunt resistance.蟾蜍膀胱和结肠的跨上皮电流-电压关系。ENaA和分流电阻的估计值。
Biophys J. 1979 Sep;27(3):371-92. doi: 10.1016/S0006-3495(79)85224-8.
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Passive electrical properties of toad urinary bladder epithelium. Intercellular electrical coupling and transepithelial cellular and shunt conductances.蟾蜍膀胱上皮的被动电特性。细胞间电偶联以及跨上皮细胞和旁路电导。
J Gen Physiol. 1974 Jul;64(1):1-25. doi: 10.1085/jgp.64.1.1.
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Regulation of epithelial Na+ permeability by protein kinase C is tissue specific.蛋白激酶C对上皮细胞钠离子通透性的调节具有组织特异性。
J Membr Biol. 1996 Aug;152(3):207-15. doi: 10.1007/s002329900098.

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3
Protocol-dependence of equivalent circuit parameters of toad urinary bladder.蟾蜍膀胱等效电路参数的实验方案依赖性

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Edge damage effect in in vitro frog skin preparations.体外青蛙皮肤制剂中的边缘损伤效应。
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