Erlij D
Pflugers Arch. 1976 Jun 29;364(1):91-3. doi: 10.1007/BF01062917.
A simple method for the routine mounting of epithelia which greatly reduces or eliminates faulty sealing at the edge is described. The values of potential and resistance observed in the frog skin and toad urinary bladder are comparable to those found with more complicated methods designed to minimize edge damage. In agreement with observations in other epithelia, a direct relationship between short circuit and conductance is observed for frog skin and toad urinary bladder, implying that the relationship is a general feature of tight epithelia. Furthermore, the high values of transepithelial resistance imply that the lower limit for the paracellular shunt pathway resistance should be reset upwards.
本文描述了一种用于常规上皮组织固定的简单方法,该方法可极大减少或消除边缘处的密封故障。在蛙皮和蟾蜍膀胱中观察到的电位和电阻值,与使用旨在最小化边缘损伤的更复杂方法所获得的值相当。与在其他上皮组织中的观察结果一致,在蛙皮和蟾蜍膀胱中观察到短路与电导之间存在直接关系,这意味着这种关系是紧密上皮组织的一个普遍特征。此外,跨上皮电阻的高值意味着细胞旁分流途径电阻的下限应向上重新设定。