Garty H
J Membr Biol. 1984;77(3):213-22. doi: 10.1007/BF01870570.
Exposure of the mucosal side of toad (Bufo bufo) urinary bladder and frog (Rana ridibunda) skin to the polyene ionophore nystatin, resulted in stable preparations in which the apical resistance was negligible compared to the basolateral resistance. The preparations support passive K currents in both directions and an amiloride-insensitive Na current in the apical-serosal direction which is blocked by ouabain. The nystatin-treated toad bladder was used to study the electrical properties of the basolateral membrane by means of current-voltage curves recorded transepithelially. The K current showed strong rectification at cellular potentials negative with respect to the interstitial space. The ouabain-sensitive current increased with membrane voltage at negative voltages but saturated above +20 mV.
将蟾蜍（Bufo bufo）膀胱黏膜侧和青蛙（Rana ridibunda）皮肤暴露于多烯离子载体制霉菌素后，得到了稳定的标本，其中顶端电阻与基底外侧电阻相比可忽略不计。这些标本支持双向的被动钾电流以及顶端 - 浆膜方向的对氨氯地平不敏感的钠电流，该钠电流可被哇巴因阻断。用制霉菌素处理过的蟾蜍膀胱通过上皮记录的电流 - 电压曲线来研究基底外侧膜的电学特性。钾电流在相对于细胞间隙为负的细胞电位时表现出强烈的整流作用。哇巴因敏感电流在负电压时随膜电压增加，但在高于 +20 mV 时达到饱和。
