Girod de Bentzmann S, Pierrot D, Fuchey C, Zahm J M, Morançais J L, Puchelle E
INSERM U 314, CHR Maison Blanche, Reims, France.
Eur Respir J. 1993 Sep;6(8):1156-61.
We have previously shown that a decreased level of phosphatidylglycerol in cystic fibrosis (CF) respiratory mucus is partly responsible for its marked adhesiveness and stickiness, which impair mucus transport, and that distearoyl phosphatidylglycerol (DSPG) was the most efficient form of phosphatidylglycerol in the enhancement of respiratory mucus clearance. The aim of our study was to analyse the effect of distearoyl phosphatidylglycerol liposomes on the transport by cough and cilia of cystic fibrosis respiratory mucus. The surface and transport properties of mucus were measured: 1) on native cystic fibrosis mucus; 2) on cystic fibrosis mucus complemented with DSPG liposomes at a non-cytotoxic concentration; and 3) on cystic fibrosis mucus complemented with water. The work of adhesion of cystic fibrosis mucus was significantly decreased by DSPG liposomes, but not by water. For mucociliary transport, the cystic fibrosis mucus was transported at a higher rate with DSPG liposomes and water compared to native cystic fibrosis mucus. The cough clearance of cystic fibrosis respiratory mucus was significantly improved in the presence of DSPG and water, but the effect was more pronounced with DSPG liposomes than with water. We conclude that the use of DSPG liposomes as a lubricating agent proves to be an interesting therapeutic approach for improving the cough and mucociliary transport in cystic fibrosis patients.
我们之前已经表明，囊性纤维化（CF）呼吸道黏液中磷脂酰甘油水平降低是其显著黏附性和黏性的部分原因，而这种黏附性和黏性会损害黏液运输，并且二硬脂酰磷脂酰甘油（DSPG）是增强呼吸道黏液清除最有效的磷脂酰甘油形式。我们研究的目的是分析二硬脂酰磷脂酰甘油脂质体对囊性纤维化呼吸道黏液咳嗽和纤毛运输的影响。测量了黏液的表面和运输特性：1）在天然囊性纤维化黏液上；2）在补充了非细胞毒性浓度DSPG脂质体的囊性纤维化黏液上；3）在补充了水的囊性纤维化黏液上。DSPG脂质体可显著降低囊性纤维化黏液的黏附功，但水则不能。对于黏液纤毛运输，与天然囊性纤维化黏液相比，补充了DSPG脂质体和水的囊性纤维化黏液运输速率更高。在存在DSPG和水的情况下，囊性纤维化呼吸道黏液的咳嗽清除率显著提高，但DSPG脂质体的效果比水更明显。我们得出结论，使用DSPG脂质体作为润滑剂被证明是一种改善囊性纤维化患者咳嗽和黏液纤毛运输的有趣治疗方法。
