Girod de Bentzmann S, Bajolet-Laudinat O, Dupuit F, Pierrot D, Fuchey C, Plotkowski M C, Puchelle E
Institut National de la Santé et de la Recherche Médicale Unité 314, Hôpital Maison Blanche, Reims, France.
Infect Immun. 1994 Feb;62(2):704-8. doi: 10.1128/iai.62.2.704-708.1994.
The ability of phosphatidylglycerol (DSPG) liposomes to prevent adherence of Pseudomonas aeruginosa to primary cultures of non-cystic fibrosis (CF) and delta F508 homozygous CF human respiratory epithelium was studied. The culture model was characterized by the simultaneous presence of various cellular phenotypes: well-differentiated respiratory epithelial cells, ciliated and nonciliated cells, and migrating cells which can be assimilated into a regenerating epithelium after injury. DSPG liposomes significantly decreased the binding of P. aeruginosa to migrating cells of both non-CF and delta F508 homozygous CF cultures compared with control cultures (35.5 x 10(-3) +/- 8.1 x 10(-3) bacteria per micron 2 versus 23.9 x 10(-3) +/- 2.5 x 10(-3); P < 0.01 for non-CF cultures and 88.8 x 10(-3) +/- 17.2 x 10(-3) bacteria per micron 2 versus 29.1 x 10(-3) +/- 0.6 x 10(-3), P < 0.001 for CF cultures). After treatment with DSPG liposomes, the size of P. aeruginosa aggregates bound to migrating cells in both non-CF cultures and delta F508 homozygous CF cultures was significantly decreased (14.4 +/- 3 bacteria per aggregate versus 11.9 +/- 2.5 bacteria per aggregate [P < 0.05] and 29.9 +/- 8.4 bacteria per aggregate versus 17.3 +/- 2.3 bacteria per aggregate [P < 0.01], respectively). Moreover, the control cultures were characterized by a differential P. aeruginosa adherence according to both the cellular phenotype and the mutation. The migrating cells bound more bacteria than the stationary cells of both non-CF and delta F508 homozygous CF cultures. The CF migrating cells bound significantly more bacteria than the non-CF migrating cells (88.8 x 10(-3) +/- 17.2 x 10(-3) bacteria per microns 2 versus 35.5 x 10(-3) +/- 8.1 x 10(-3) bacteria per micron 2, P < 0.001). These results suggest that DSPG liposomes are able to decrease P. aeruginosa adherence to CF and non-CF respiratory epithelium, particularly to migrating cells, which mimic a regenerating epithelium after injury. DSPG liposomes could also represent a hydrophobic barrier limiting the deleterious action of P. aeruginosa exoproducts.
研究了磷脂酰甘油（DSPG）脂质体预防铜绿假单胞菌黏附于非囊性纤维化（CF）和ΔF508纯合CF人呼吸道上皮原代培养物的能力。该培养模型的特征是同时存在多种细胞表型：分化良好的呼吸道上皮细胞、有纤毛和无纤毛细胞，以及损伤后可融入再生上皮的迁移细胞。与对照培养物相比，DSPG脂质体显著降低了铜绿假单胞菌对非CF和ΔF508纯合CF培养物迁移细胞的黏附（非CF培养物中每平方微米35.5×10⁻³±8.1×10⁻³个细菌，而对照为23.9×10⁻³±2.5×10⁻³个细菌；P<0.01；CF培养物中每平方微米88.8×10⁻³±17.2×10⁻³个细菌，而对照为29.1×10⁻³±0.6×10⁻³个细菌，P<0.001）。用DSPG脂质体处理后，非CF培养物和ΔF508纯合CF培养物中与迁移细胞结合的铜绿假单胞菌聚集体大小均显著减小（非CF培养物中每个聚集体14.4±3个细菌，对照为11.9±2.5个细菌[P<0.05]；CF培养物中每个聚集体29.9±8.4个细菌，对照为17.3±2.3个细菌[P<0.01]）。此外，对照培养物的特征是根据细胞表型和突变存在不同的铜绿假单胞菌黏附情况。非CF和ΔF508纯合CF培养物的迁移细胞比静止细胞结合更多细菌。CF迁移细胞比非CF迁移细胞结合的细菌显著更多（每平方微米88.8×10⁻³±17.2×10⁻³个细菌，而对照为每平方微米35.5×10⁻³±8.1×10⁻³个细菌，P<0.001）。这些结果表明，DSPG脂质体能够降低铜绿假单胞菌对CF和非CF呼吸道上皮的黏附，特别是对迁移细胞的黏附，这些迁移细胞模拟损伤后的再生上皮。DSPG脂质体也可能代表一种疏水屏障，限制铜绿假单胞菌外毒素的有害作用。
