淋巴细胞刺激：快速多参数分析
Lymphocyte stimulation: a rapid multiparameter analysis.
作者信息
Darzynkiewicz Z, Traganos F, Sharpless T, Melamed M R
出版信息
Proc Natl Acad Sci U S A. 1976 Aug;73(8):2881-4. doi: 10.1073/pnas.73.8.2881.
Abstract
Several parameters of stimulation of individual lymphocytes are measured simultaneously by flow-cytofluorometry after differential staining of cellular DNA and RNA with the metachromatic fluorescent dye acridine orange. The method provides a means of analyzing the progression of stimulated cells through the cell cycle (G1, S and G2 + M), in addition to measuring the accumulation of RNA per cell; the RNA parameter is useful in distinguishing G1 from G0 cells. The multiparameter analysis may be of unique value in discriminating cases where the transcriptional and proliferative responses of lymphocytes to stimulants are not correlated.
摘要
在用异染荧光染料吖啶橙对细胞DNA和RNA进行差异染色后，通过流式细胞荧光术同时测量单个淋巴细胞的几个刺激参数。该方法除了可测量每个细胞中RNA的积累外，还提供了一种分析受刺激细胞在细胞周期（G1、S和G2+M）中的进展情况的方法；RNA参数有助于区分G1期细胞和G0期细胞。在区分淋巴细胞对刺激物的转录反应和增殖反应不相关的情况时，多参数分析可能具有独特的价值。
相似文献
1
Lymphocyte stimulation: a rapid multiparameter analysis.淋巴细胞刺激：快速多参数分析
Proc Natl Acad Sci U S A. 1976 Aug;73(8):2881-4. doi: 10.1073/pnas.73.8.2881.
2
Rapid multiparameter analysis of cell stimulation in mixed lymphocyte culture reactions.混合淋巴细胞培养反应中细胞刺激的快速多参数分析
J Histochem Cytochem. 1977 Jul;25(7):881-7. doi: 10.1177/25.7.142787.
3
Conformation of RNA in situ as studied by acridine orange staining and automated cytofluorometry.通过吖啶橙染色和自动细胞荧光测定法研究RNA的原位构象。
Exp Cell Res. 1975 Oct 1;95(1):143-53. doi: 10.1016/0014-4827(75)90619-9.
4
Cell cycle-related changes in nuclear chromatin of stimulated lymphocytes as measured by flow cytometry.通过流式细胞术测量刺激淋巴细胞的核染色质中与细胞周期相关的变化。
Cancer Res. 1977 Dec;37(12):4635-40.
5
Bivariate analysis of cellular DNA versus RNA content by laser scanning cytometry using the product of signal subtraction (differential fluorescence) as a separate parameter.通过激光扫描细胞术对细胞DNA与RNA含量进行双变量分析，使用信号减法的产物（差异荧光）作为一个单独参数。
Cytometry. 2001 Sep 1;45(1):73-8. doi: 10.1002/1097-0320(20010901)45:1<73::aid-cyto1146>3.0.co;2-a.
6
Simultaneous staining of ribonucleic and deoxyribonucleic acids in unfixed cells using acridine orange in a flow cytofluorometric system.在流动细胞荧光测定系统中使用吖啶橙对未固定细胞中的核糖核酸和脱氧核糖核酸进行同时染色。
J Histochem Cytochem. 1977 Jan;25(1):46-56. doi: 10.1177/25.1.64567.
7
Discrimination of cycling and non-cycling lymphocytes by BUdR-suppressed acridine orange fluorescence in a flow cytometric system.在流式细胞术系统中通过5-溴脱氧尿苷（BUdR）抑制的吖啶橙荧光鉴别循环淋巴细胞和非循环淋巴细胞。
Exp Cell Res. 1978 Aug;115(1):31-5. doi: 10.1016/0014-4827(78)90398-1.
8
Cytofluorometric studies on conformation of nucleic acids in situ. I. Restriction of acridine orange binding by chromatin proteins.原位核酸构象的细胞荧光分析研究。I. 染色质蛋白对吖啶橙结合的限制
J Histochem Cytochem. 1976 Jan;24(1):40-8. doi: 10.1177/24.1.1254934.
9
Flow cytometric analysis of RNA content in different cell populations using pyronin Y and methyl green.使用派洛宁Y和甲基绿对不同细胞群体中的RNA含量进行流式细胞术分析。
Cytometry. 1982 Jul;3(1):28-35. doi: 10.1002/cyto.990030108.
10
The ratio of RNA to total nucleic acid content as a quantitative measure of unbalanced cell growth.作为细胞生长失衡定量指标的RNA与总核酸含量之比。
Cytometry. 1982 Jan;2(4):212-8. doi: 10.1002/cyto.990020403.
引用本文的文献
1
Factors to Consider for the Correct Use of γH2AX in the Evaluation of DNA Double-Strand Breaks Damage Caused by Ionizing Radiation.在评估电离辐射引起的DNA双链断裂损伤时正确使用γH2AX需考虑的因素
Cancers (Basel). 2022 Dec 15;14(24):6204. doi: 10.3390/cancers14246204.
2
Establishment of a fluorescent reporter of RNA-polymerase II activity to identify dormant cells.建立荧光报告基因检测 RNA 聚合酶 II 活性以鉴定休眠细胞
Nat Commun. 2021 Jun 3;12(1):3318. doi: 10.1038/s41467-021-23580-4.
3
Lufenuron induces reproductive toxicity and genotoxic effects in pregnant albino rats and their fetuses.伏虫隆对怀孕的白化孕鼠及其胎儿具有生殖毒性和遗传毒性作用。
Sci Rep. 2020 Nov 11;10(1):19544. doi: 10.1038/s41598-020-76638-6.
4
Circulating CD3HLA-DR Extracellular Vesicles as a Marker for Th1/Tc1-Type Immune Responses.循环 CD3HLA-DR 细胞外囊泡作为 Th1/Tc1 型免疫应答的标志物。
J Immunol Res. 2019 May 8;2019:6720819. doi: 10.1155/2019/6720819. eCollection 2019.
5
Initiation and termination of DNA replication during S phase in relation to cyclins D1, E and A, p21WAF1, Cdt1 and the p12 subunit of DNA polymerase δ revealed in individual cells by cytometry.通过细胞计数法在单个细胞中揭示的S期DNA复制的起始和终止与细胞周期蛋白D1、E和A、p21WAF1、Cdt1以及DNA聚合酶δ的p12亚基的关系。
Oncotarget. 2015 May 20;6(14):11735-50. doi: 10.18632/oncotarget.4149.
6
Expression of the p12 subunit of human DNA polymerase δ (Pol δ), CDK inhibitor p21(WAF1), Cdt1, cyclin A, PCNA and Ki-67 in relation to DNA replication in individual cells.人DNA聚合酶δ（Pol δ）的p12亚基、细胞周期蛋白依赖性激酶抑制剂p21（WAF1）、Cdt1、细胞周期蛋白A、增殖细胞核抗原（PCNA）和Ki-67在单个细胞中与DNA复制相关的表达。
Cell Cycle. 2014;13(22):3529-40. doi: 10.4161/15384101.2014.958910.
7
Polarized neural stem cells derived from adult bone marrow stromal cells develop a rosette-like structure.由成体骨髓基质细胞诱导分化而来的神经干细胞呈类玫瑰花结样结构。
In Vitro Cell Dev Biol Anim. 2013 Sep;49(8):638-52. doi: 10.1007/s11626-013-9628-y. Epub 2013 Jun 15.
8
Multiparameter flow cytometry: advances in high resolution analysis.多参数流式细胞术：高分辨率分析的进展。
Immune Netw. 2013 Apr;13(2):43-54. doi: 10.4110/in.2013.13.2.43. Epub 2013 Apr 30.
9
Potential anti-aging agents suppress the level of constitutive mTOR- and DNA damage- signaling.潜在的抗衰老剂可抑制组成性mTOR和DNA损伤信号传导的水平。
Aging (Albany NY). 2012 Dec;4(12):952-65. doi: 10.18632/aging.100521.
10
Cycling into future: mass cytometry for the cell-cycle analysis.迈向未来的循环：用于细胞周期分析的质谱流式细胞术
Cytometry A. 2012 Jul;81(7):546-8. doi: 10.1002/cyto.a.22080. Epub 2012 Jun 12.
本文引用的文献
1
AGGREGATION OF DYES BOUND TO POLYANIONS.与聚阴离子结合的染料聚集
Proc Natl Acad Sci U S A. 1959 Jul;45(7):944-52. doi: 10.1073/pnas.45.7.944.
2
Phytohemagglutinin: an initiator of mitosis in cultures of normal human leukocytes.植物血凝素：正常人白细胞培养中细胞有丝分裂的启动剂。
Cancer Res. 1960 May;20:462-6.
3
The structure of the DNA-acridine complex.DNA-吖啶复合物的结构。
Proc Natl Acad Sci U S A. 1963 Jan 15;49(1):94-102. doi: 10.1073/pnas.49.1.94.
4
Use of acridine orange in lymphocyte transformation test.吖啶橙在淋巴细胞转化试验中的应用。
Proc Soc Exp Biol Med. 1969 Sep;131(4):1168-70. doi: 10.3181/00379727-131-34062.
5
In vitro development of plasma cells from lymphocytes following pokeweed mitogen stimulation: a fine structural study.商陆有丝分裂原刺激后淋巴细胞体外发育为浆细胞的精细结构研究
Exp Cell Res. 1969 Feb;54(2):277-9. doi: 10.1016/0014-4827(69)90252-3.
6
Metal ions and ribosomal conformation.金属离子与核糖体构象
Biochim Biophys Acta. 1969 Nov 19;195(1):76-86. doi: 10.1016/0005-2787(69)90604-2.
7
"Transcriptional mapping". I. Introduction to the method and the use of actinomycin D as a transcriptional mapping agent.“转录图谱分析”。一、该方法简介以及放线菌素D作为转录图谱分析试剂的应用
Proc Natl Acad Sci U S A. 1969 Jun;63(2):532-9. doi: 10.1073/pnas.63.2.532.
8
Use of ethidium bromide for separation and determination of nucleic acids of various conformational forms and measurement of their associated enzymes.溴化乙锭用于分离和测定各种构象形式的核酸及其相关酶的活性。
Methods Biochem Anal. 1971;20:41-86. doi: 10.1002/9780470110393.ch2.
9
Variability of the time at which PHA-stimulated lymphocytes initiate DNA synthesis.PHA刺激的淋巴细胞启动DNA合成的时间变异性。
Exp Cell Res. 1973 Mar 30;78(1):201-8. doi: 10.1016/0014-4827(73)90055-4.
10
Discussion paper: studies on the differentiation within the immune system.讨论文件：免疫系统内的分化研究。
Ann N Y Acad Sci. 1973 May 31;207:57-72. doi: 10.1111/j.1749-6632.1973.tb47476.x.
Zotero 插件