“转录图谱分析”。一、该方法简介以及放线菌素D作为转录图谱分析试剂的应用
"Transcriptional mapping". I. Introduction to the method and the use of actinomycin D as a transcriptional mapping agent.
作者信息
Bleyman M, Woese C
出版信息
Proc Natl Acad Sci U S A. 1969 Jun;63(2):532-9. doi: 10.1073/pnas.63.2.532.
Abstract
The transcription process can in theory be used for genetic mapping on the "intraoperonal" level. We have shown that such an approach, utilizing actinomycin D to create blocks to the travel of the transcription enzyme, is indeed feasible, and the effects of actinomycin D on the synthesis of general pulse-labeled RNA are reported herein.
摘要
转录过程理论上可用于“手术内”水平的基因定位。我们已经表明，利用放线菌素D来阻止转录酶移动的这种方法确实可行，本文报道了放线菌素D对一般脉冲标记RNA合成的影响。
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