Sheu J C, Huang G T, Chou H C, Lee P H, Wang J T, Lee H S, Chen D S
Department of Internal Medicine, National Taiwan University Hospital, College of Medicine, National Taiwan University, Taipei, Republic of China.
Gastroenterology. 1993 Nov;105(5):1471-6. doi: 10.1016/0016-5085(93)90153-4.
The clonality of hepatocellular carcinoma is still controversial. To evaluate the clonality of this cancer, mostly small multiple hepatocellular carcinomas of 18 patients were analyzed by DNA fingerprinting.
DNA from the liver tissues was digested with HinfI. After gel electrophoresis, in gel-hybridization with a synthetic oligonucleotide probe GTGGTGGTGGTGGTG was performed. The band patterns were compared among the tumors and the nontumor part. In patients positive for hepatitis B surface antigen (HBsAg), the integration pattern of hepatitis B viral DNA in liver tissues was also analyzed.
Seven of the 9 HBsAg-positive patients had tumors with different clonalities by both methods. In the remaining 9 HBsAg-negative patients, 4 had different band patterns in their tumors by DNA fingerprinting.
Multiple hepatocellular carcinomas frequently have different clonalities; this finding reiterates the importance of eliminating the underlying cause and the contributing factors of hepatocarcinogenesis.
肝细胞癌的克隆性仍存在争议。为评估该癌症的克隆性，对18例患者的大多为小的多发性肝细胞癌进行了DNA指纹分析。
用HinfI消化肝组织中的DNA。凝胶电泳后，用合成寡核苷酸探针GTGGTGGTGGTGGTG进行凝胶杂交。比较肿瘤与非肿瘤部分的条带模式。对于乙型肝炎表面抗原（HBsAg）阳性患者，还分析了肝组织中乙型肝炎病毒DNA的整合模式。
9例HBsAg阳性患者中有7例通过两种方法检测到肿瘤具有不同的克隆性。在其余9例HBsAg阴性患者中，4例通过DNA指纹分析显示其肿瘤具有不同的条带模式。
多发性肝细胞癌常具有不同的克隆性；这一发现再次强调了消除肝癌发生的潜在病因和促成因素的重要性。
