由黑曲霉的可阻遏酸性磷酸酶系统指导的异源蛋白分泌。

Heterologous protein secretion directed by a repressible acid phosphatase system of Aspergillus niger.

作者信息

MacRae W D, Buxton F P, Gwynne D I, Davies R W

机构信息

Allelix Biopharmaceuticals Inc., Mississauga, Ontario, Canada.

出版信息

Gene. 1993 Oct 15;132(2):193-8. doi: 10.1016/0378-1119(93)90195-9.

DOI:10.1016/0378-1119(93)90195-9

PMID:8224863

Abstract

A new expression-secretion system of Aspergillus niger which directs the secretion of heterologous proteins is described. The promoter and signal peptide-encoding region of the phosphate-repressible aphA gene of A. niger, when fused to the coding region of the human interferon alpha 2 (hIFN alpha 2)-encoding gene (hIFN alpha 2), drives the expression of this gene and the secretion of the hIFN alpha 2 protein. Synthesis of hIFN alpha 2 in either A. niger or A. nidulans transformants carrying these constructs was regulated by inorganic phosphate (Pi) present in the medium, so that derepression of heterologous protein expression can be attained by lowering Pi concentration.

摘要

描述了一种新的黑曲霉表达分泌系统，该系统可指导异源蛋白质的分泌。黑曲霉的磷酸盐可阻遏aphA基因的启动子和信号肽编码区，当与人类干扰素α2（hIFNα2）编码基因（hIFNα2）的编码区融合时，可驱动该基因的表达以及hIFNα2蛋白的分泌。携带这些构建体的黑曲霉或构巢曲霉转化体中hIFNα2的合成受培养基中存在的无机磷酸盐（Pi）调节，因此通过降低Pi浓度可实现异源蛋白质表达的去阻遏。