泡盛曲霉中编码植酸酶（phy）和最适pH 2.5酸性磷酸酶（aph）的基因的克隆与测序。
The cloning and sequencing of the genes encoding phytase (phy) and pH 2.5-optimum acid phosphatase (aph) from Aspergillus niger var. awamori.
作者信息
Piddington C S, Houston C S, Paloheimo M, Cantrell M, Miettinen-Oinonen A, Nevalainen H, Rambosek J
机构信息
Panlabs Incorporated, Bothell, WA 98011-8805.
出版信息
Gene. 1993 Oct 29;133(1):55-62. doi: 10.1016/0378-1119(93)90224-q.
The genes encoding phytase (EC 3.1.3.8) and pH 2.5-optimum acid phosphatase (EC 3.1.3.2) have been cloned and sequenced from Aspergillus niger var. awamori. The translated nucleotide sequences yielded polypeptides of 467 and 479 amino acids (aa) for phytase and acid phosphatase, respectively. The genes were isolated using oligodeoxyribonucleotide probes based on the aa sequences of the purified proteins. Recombinant A. niger var. awamori strains carrying additional copies of the gene sequences demonstrated elevated enzyme activities.
编码植酸酶（EC 3.1.3.8）和最适pH 2.5酸性磷酸酶（EC 3.1.3.2）的基因已从泡盛曲霉中克隆并测序。翻译后的核苷酸序列分别产生了由467个和479个氨基酸组成的植酸酶和酸性磷酸酶多肽。基于纯化蛋白的氨基酸序列，使用寡脱氧核糖核苷酸探针分离出这些基因。携带这些基因序列额外拷贝的重组泡盛曲霉菌株表现出更高的酶活性。
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