MacRae W D, Buxton F P, Sibley S, Garven S, Gwynne D I, Davies R W, Arst H N
Allelix Inc., Mississauga, Ont., Canada.
Gene. 1988 Nov 30;71(2):339-48. doi: 10.1016/0378-1119(88)90051-0.
The cloning and sequencing of an Aspergillus niger gene encoding a secreted form of phosphate-repressible acid phosphatase by complementation of a pacA (phosphate-repressible acid phosphatase) mutant of Aspergillus nidulans is described. The gene contains two introns, 201 and 265 nt in length, and codes for a 1.6-kb transcript. Both phosphate concentration and pH of the growth medium affect the level of expression of the gene in A. niger. Similar regulation is observed in A. nidulans transformants. A putative signal peptide, resembling known signal sequences of yeast, is identified.
本文描述了通过对构巢曲霉pacA（磷酸可阻遏酸性磷酸酶）突变体进行互补作用，克隆和测序黑曲霉中编码一种分泌形式的磷酸可阻遏酸性磷酸酶的基因。该基因含有两个长度分别为201和265 nt的内含子，编码一个1.6 kb的转录本。生长培养基中的磷酸盐浓度和pH值都会影响该基因在黑曲霉中的表达水平。在构巢曲霉转化体中也观察到了类似的调控。鉴定出一个类似于酵母已知信号序列的推定信号肽。
