Nishimura K, Nakayashiki T, Inokuchi H
Department of Biophysics, Faculty of Science, Kyoto University, Japan.
Gene. 1993 Oct 29;133(1):109-13. doi: 10.1016/0378-1119(93)90233-s.
Among the photoresistant revertants of the visA-deleted (hemH-deleted) strain of Escherichia coli K-12, three mutants defective in the hemE gene encoding uroporphyrinogen III decarboxylase (UPD) were identified. Using one of the mutants, we cloned and sequenced the hemE of E. coli. We found an open reading frame of 353 codons, which encoded a predicted amino acid (aa) sequence that exhibited a high degree of homology over its entire length to the aa sequence of UPD from humans and other organisms. This hemE was located at 90.3 min near the hupA gene on the linkage map of the E. coli chromosome.
在大肠杆菌K-12的visA缺失（hemH缺失）菌株的光抗性回复突变体中，鉴定出了3个在编码尿卟啉原III脱羧酶（UPD）的hemE基因中有缺陷的突变体。利用其中一个突变体，我们克隆并测序了大肠杆菌的hemE。我们发现了一个由353个密码子组成的开放阅读框，其编码的预测氨基酸（aa）序列在全长上与人类和其他生物的UPD的aa序列具有高度同源性。这个hemE位于大肠杆菌染色体连锁图谱上hupA基因附近90.3分钟处。
