Pulkkinen M O, Kivikoski A I, Nevalainen T J
Department of Obstetrics and Gynecology, University of Turku, Finland.
Gynecol Obstet Invest. 1993;36(2):96-101. doi: 10.1159/000292604.
Phospholipase A2 groups I (pancreatic) and II (synovial) could be a link between local and systemic changes in pregnancy, reflected in catalytic activity. We studied whether normal pregnancy, preeclampsia, preterm labor and four other diseases have processes involving serum phospholipase A2s. Pancreatic and synovial-type phospholipase A2 were measured in the serum of 59 normal pregnant women and 89 patients with pathological pregnancy by newly developed time-resolved fluoroimmunoassays, and the catalytic activity by a radiochemical method using micellar phosphatidylcholine as substrate. During pregnancy weeks 6-14, synovial-type phospholipase A2 and catalytic activity were elevated 2- to 4-fold, but at 37 weeks values were normal. Pregnancy-induced hypertensive diseases increased by 4- to 10-fold the concentration of synovial-type phospholipase A2, reflected in catalytic activity. In 8 out of 14 cases, the enzyme was increased if the fetus was to be delivered prematurely. The enzymes studied remained within the reference interval in cases of hepatogestosis, fetal asphyxia, diabetes and twin pregnancy. Newly developed specific immunoassays for measuring different types of phospholipase A2 in serum can provide insights for clinical follow-up.
I型（胰腺型）和II型（滑膜型）磷脂酶A2可能是妊娠中局部和全身变化之间的一个联系，这在催化活性中有所体现。我们研究了正常妊娠、先兆子痫、早产以及其他四种疾病是否存在涉及血清磷脂酶A2的过程。通过新开发的时间分辨荧光免疫分析法测定了59名正常孕妇和89名病理妊娠患者血清中的胰腺型和滑膜型磷脂酶A2，并采用以胶束磷脂酰胆碱为底物的放射化学方法测定了催化活性。在妊娠第6至14周期间，滑膜型磷脂酶A2和催化活性升高了2至4倍，但在37周时数值正常。妊娠高血压疾病使滑膜型磷脂酶A2的浓度升高了4至10倍，这在催化活性中得到体现。在14例中有8例，如果胎儿将早产，该酶会升高。在肝内胆汁淤积症、胎儿窒息、糖尿病和双胎妊娠病例中，所研究的酶仍在参考区间内。新开发的用于测定血清中不同类型磷脂酶A2的特异性免疫分析法可为临床随访提供见解。
