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使用酶联免疫吸附测定法(ELISA)检测供屠宰的自然感染旋毛虫的猪(作者译)

[Detection of Trichinella spiralis infection in spontaneously infected pigs intended for slaughter using the enzyme-linked immunosorbent assay (ELISA) (author's transl)].

作者信息

Van Knapen F, Framstad K, Ruitenberg E J

出版信息

Tijdschr Diergeneeskd. 1976 Sep 1;101(17):952-6.

PMID:822533
Abstract

In a study of seventy-seven pigs intended for saughter, from two farms on which Trichinella infections had previously been detected by trichinoscopy, the sensitivity of the enzyme-linked immunosorbent assay (ELISA) was compared with that of two direct (trichinoscopy and digestion) and three indirect (serological) methods of detection (fluorescent antibody technique, counter-electrophoresis and Ouchterlony's agar gel diffusion method). In five out of seventy-seven pigs, the digestion procedure revealed the presence of larvae of Trichinella spiralis in the diaphragm. Four of these pigs showed a very high degree of infection, whereas one showed a very low degree of infection. Infection was shown to be present in all five infected pigs using the ELISA method, whereas the findings were only positive in the four severely infected pigs when trichinoscopy, the fluorescent antibody technique, counter-electrophoresis and Ouchterlony's method were used. The ELISA was sufficiently sensitive to detect Trichinella infection even in those pigs which showed a low degree of infection.

摘要

在一项针对77头准备屠宰的猪的研究中,这些猪来自之前通过旋毛虫镜检检测出旋毛虫感染的两个农场,将酶联免疫吸附测定(ELISA)的灵敏度与两种直接检测方法(旋毛虫镜检和消化法)以及三种间接检测方法(血清学方法)(荧光抗体技术、对流免疫电泳和双向琼脂扩散法)进行了比较。在77头猪中,有5头猪的消化程序显示膈肌中有旋毛虫幼虫。其中4头猪感染程度很高,而1头猪感染程度很低。使用ELISA方法显示所有5头感染猪均存在感染,而当使用旋毛虫镜检、荧光抗体技术、对流免疫电泳和双向琼脂扩散法时,仅在4头严重感染的猪中检测结果呈阳性。ELISA足够灵敏,即使在感染程度低的猪中也能检测出旋毛虫感染。

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