两种血清因子，维生素D3结合蛋白和白蛋白，对炎症引发的巨噬细胞活化的调节作用。
Regulation of inflammation-primed activation of macrophages by two serum factors, vitamin D3-binding protein and albumin.
作者信息
Yamamoto N, Kumashiro R, Yamamoto M, Willett N P, Lindsay D D
机构信息
Department of Biochemistry, Temple University School of Medicine, Philadelphia, Pennsylvania 19140.
出版信息
Infect Immun. 1993 Dec;61(12):5388-91. doi: 10.1128/iai.61.12.5388-5391.1993.
Abstract
A very small amount (0.0005 to 0.001%) of an ammonium sulfate [50% saturated (NH4)2SO4]-precipitable protein fraction of alpha 2-globulin efficiently supported inflammation-primed activation of macrophages. This fraction contains vitamin D3-binding protein essential for macrophage activation. Comparative macrophage activation studies with fetal calf serum, alpha 2-globulin fraction, 50% (NH4)2SO4 precipitate, and purified bovine vitamin D3-binding protein revealed that fetal calf serum and alpha 2-globulin fraction appear to contain an inhibitor for macrophage activation while ammonium sulfate precipitate contains no inhibitor. This inhibitor was found to be serum albumin. When bovine serum albumin (25 micrograms/ml) was added to a medium supplemented with 0.0005 to 0.05% (NH4)2SO4 precipitate or 1 to 10 ng of vitamin D3-binding protein per ml, activation of macrophages was inhibited.
摘要
α2球蛋白的硫酸铵[50%饱和(NH4)2SO4]可沉淀蛋白部分中极少量(0.0005%至0.001%)能有效支持炎症引发的巨噬细胞激活。该部分含有巨噬细胞激活所必需的维生素D3结合蛋白。用胎牛血清、α2球蛋白部分、50%(NH4)2SO4沉淀物和纯化的牛维生素D3结合蛋白进行的巨噬细胞激活比较研究表明，胎牛血清和α2球蛋白部分似乎含有巨噬细胞激活抑制剂，而硫酸铵沉淀物不含抑制剂。发现该抑制剂为血清白蛋白。当将牛血清白蛋白(25微克/毫升)添加到补充有0.0005%至0.05%(NH4)2SO4沉淀物或每毫升1至10纳克维生素D3结合蛋白的培养基中时，巨噬细胞的激活受到抑制。
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