Kimoto T, Namba M, Ueki A, Hyodoh F
Virchows Arch A Pathol Anat Histol. 1976 Aug 19;371(1):15-26. doi: 10.1007/BF00433711.
The peripheral blood of an acute myelomonocytic leukemia patient has been cultured for 16 months. The culture is at present at the 140th population doupling level. The cultured cells have the characteristics of so-called lymphoblastoid cells and proliferate actively as individual cells in small clusters, or in large clumps consisting of large mononuclear cells. Some of these cells appeare to be lymphoid, but the majority are immature mononuclear cells with a tendency to lobulate. They gave a weakly positive peroxidase reaction at the beginning of cultivation, and have given a strongly positive esterase reaction persistently. The cytoplasm shows ciliary or tail-like projections as the cell matures. Complement (C3) receptor and IgG receptors are found on the cell surface, and active phagocytosis is mannifest. Colloidal iron particles or viable red blood cells attached to the cell membrane suggesting possible differentiation to reticulum cells or macrophages. The cultured cells are mostly diploid but some cells show chromosome abnormality. Herpes type virus was foun in the nucleus, cytoplasma and on the cell membrane. The transplanatation of cultured cells to the cheek pouch of hamsters produced small tumors with histological findings resembling reticulum cell sarcoma.
一名急性粒单核细胞白血病患者的外周血已培养16个月。目前培养处于第140个群体倍增水平。培养的细胞具有所谓淋巴母细胞样细胞的特征，以单个细胞小簇或由大单核细胞组成的大团块形式活跃增殖。这些细胞中有些似乎是淋巴细胞，但大多数是有分叶倾向的未成熟单核细胞。培养开始时它们过氧化物酶反应呈弱阳性，而酯酶反应持续呈强阳性。随着细胞成熟，细胞质显示出纤毛状或尾状突起。细胞表面发现有补体（C3）受体和IgG受体，且表现出活跃的吞噬作用。附着在细胞膜上的胶体铁颗粒或活红细胞表明可能分化为网状细胞或巨噬细胞。培养的细胞大多是二倍体，但有些细胞显示出染色体异常。在细胞核、细胞质和细胞膜上发现了疱疹病毒。将培养细胞移植到仓鼠颊囊产生了小肿瘤，组织学检查结果类似于网状细胞肉瘤。
