Ohno H, Doi S, Fukuhara S, Yamasowa M, Kannagi M, Ishikura H, Nishikori M, Uchino H
Int J Cancer. 1986 May 15;37(5):761-7. doi: 10.1002/ijc.2910370518.
A myeloid leukemia cell line (TK-1) was established from the peripheral blood of a patient with lymphoblastic lymphoma whose leukemia cells were composed of T-lymphoblasts and immature myeloid cells. The established TK-1 cell line consisted of immature myeloid cells with heavy azurophilic granulation in the cytoplasm. The TK-1 cells were positive for peroxidase, and exhibited a strong positive reaction for alpha-naphthyl acetate esterase and naphthol AS-D chloroacetate esterase. The cells were weakly positive for Fc gamma-receptors, showed no phagocytosis and did not reduce NBT. With the treatment of 1 alpha, 25-dihydroxy-vitamin D3, they exhibited morphological and functional differentiation. The TK-1 cell line contained normal diploid cells and pseudodiploid cells, and the two populations were successfully cloned; the clone with a normal karyotype was designated the TK-1D cell line, and the clone with a pseudodiploid karyotype, which had a translocation involving chromosomes 14, 17 and one other chromosome, was designated the TK-1B cell line. These cloned cells lacked Epstein-Barr virus nuclear antigens and had almost the same myelomonocytic characteristics as the parent TK-1 cells. The breakpoint of chromosome 17 involved in the translocation of the pseudodiploid cells was identified to be a band 17q23.
一种髓系白血病细胞系（TK - 1）是从一名淋巴细胞淋巴瘤患者的外周血中建立的，该患者的白血病细胞由T淋巴细胞和未成熟髓细胞组成。所建立的TK - 1细胞系由细胞质中具有粗大嗜天青颗粒的未成熟髓细胞组成。TK - 1细胞过氧化物酶呈阳性，对α - 萘乙酸酯酶和萘酚AS - D氯乙酸酯酶呈强阳性反应。细胞对Fcγ受体呈弱阳性，无吞噬作用，不还原NBT。用1α，25 - 二羟基维生素D3处理后，它们表现出形态和功能分化。TK - 1细胞系包含正常二倍体细胞和假二倍体细胞，并且这两个群体成功克隆；具有正常核型的克隆被命名为TK - 1D细胞系，具有假二倍体核型且涉及14号、17号染色体和另一条染色体易位的克隆被命名为TK - 1B细胞系。这些克隆细胞缺乏EB病毒核抗原，并且具有与亲本TK - 1细胞几乎相同的髓单核细胞特征。假二倍体细胞易位中涉及的17号染色体的断点被确定为17q23带。
