Klein E, Ben-Bassat H, Neumann H, Ralph P, Zeuthen J, Polliack A, Vánky F
Int J Cancer. 1976 Oct 15;18(4):421-31. doi: 10.1002/ijc.2910180405.
The K562 cell line derived from a CML patient in blast crisis was examined for properties of B and T lymphocytes and cell lines. K562 lacks the B markers of immunoglobulins, Epstein-Barr virus (EBV) genome and associated nuclear antigen, and receptors for EBV. A low proportion of cells from rosettes with sheep erythrocytes, the frequency of which is considerably increased after neuraminidase treatment. Unlike B lines but like T lines, K562 cells are lysed rapidly by C'/Fc receptor-positive human blood leukocytes and do not stimulate MLC reactions. On the other hand, K562 lacks T antigen, high radiosensitivity and sensitivity to growth inhibition by thymidine. The cells do not contain N-APase, an enzyme found in all lines derived from lymphoid cells and in lymphoproliferative diseases. By scanning electron microscopy, K562 cells were seen to be rounded and relatively smooth, with small numbers of short microvilli resembling undifferentiated leukemic cells. A few cells had narrow ridge-like profiles and small ruffles similar to granulocytic leukemic cells. K562 is strongly positive for immunoglobuln Fc receptors and pinocytosis, but does not phagocytose or mediate antibody-dependent phagocytosis or cytolysis. Among histochemical stains, K562 is positive for esterase, lipid, and acid phosphatase. There seems to be no doubt that K562 is not a B cell line. While it has some T cell properties, these are not exclusive. Some of its characteristics indicate that it is probably not lymphoid. Due to its low level of differentiation, its nature cannot be stated with certainty. On the basis of the possible presence of the cellular marker of chronic myeloid leukemia, the Ph chromosome, it may be regarded as belonging to the granulocytic series of cells.
对源自一名处于急变期的慢性粒细胞白血病（CML）患者的K562细胞系进行了B淋巴细胞、T淋巴细胞及细胞系特性检测。K562缺乏免疫球蛋白的B标志物、EB病毒（EBV）基因组及相关核抗原，也缺乏EBV受体。与绵羊红细胞形成玫瑰花结的细胞比例较低，经神经氨酸酶处理后该比例显著增加。与B细胞系不同，但与T细胞系相似，K562细胞可被补体/C'Fc受体阳性的人血白细胞迅速裂解，且不刺激混合淋巴细胞培养（MLC）反应。另一方面，K562缺乏T抗原、高放射敏感性以及对胸腺嘧啶生长抑制的敏感性。这些细胞不含N - 酸性磷酸酶，该酶存在于所有源自淋巴细胞的细胞系及淋巴增殖性疾病中。通过扫描电子显微镜观察，K562细胞呈圆形且相对光滑，有少量短微绒毛，类似未分化的白血病细胞。少数细胞有类似粒细胞白血病细胞的窄嵴状轮廓和小褶皱。K562对免疫球蛋白Fc受体和胞饮作用呈强阳性，但不进行吞噬或介导抗体依赖性吞噬或细胞溶解。在组织化学染色中，K562对酯酶、脂质和酸性磷酸酶呈阳性。毫无疑问，K562不是B细胞系。虽然它具有一些T细胞特性，但并非独特。其一些特征表明它可能不是淋巴细胞。由于其低分化水平，其性质尚不能确定。基于慢性粒细胞白血病细胞标志物Ph染色体可能存在的情况，它可被视为属于粒细胞系列细胞。
