Derbin M I, Mikhaĭlov V V, Tarumov V S, Garin N S, Fedorova N V
Zh Mikrobiol Epidemiol Immunobiol. 1976(3):76-81.
The authors present the results of studies concerning the choice of nutrient bases and elaboration of a medium for obtaining anthrax protective antigen providing an active preparation. Filtrates of crude cultures obtained on the nutrient medium prepared of 3% hydrochloric hydrolyzate of fish flour, corn extract, glucose, sodium bicarbonate and mineral salts proved to be the most active. Tests for determination of usefulness of the culture fluids with the maximum content in them of the protective antigen are recommended.
作者介绍了有关营养基础选择的研究结果，并阐述了一种用于获得炭疽保护性抗原的培养基，以提供一种活性制剂。在由3%鱼粉盐酸水解产物、玉米提取物、葡萄糖、碳酸氢钠和矿物盐制备的营养培养基上获得的粗培养物滤液被证明是最具活性的。建议对保护性抗原含量最高的培养液进行有效性测试。
