Shliakhov E N, Gruz E V, Burdenko T A, Moroz L V
Zh Mikrobiol Epidemiol Immunobiol. 1977 Sep(9):41-5.
The results of examination of immunological properties of the preparations of anthrax protective antigen on laboratory animals (guinea pigs) confirmed the efficacy of using the lactic-peptone medium for obtaining the anthrax protective antigen. Incubation for 18 hours at 37 degrees C of the strain-producer (STI-1) and a double immunization scheme with the antigen obtained proved to be the most rational conditions for inducing the immunological response in the vaccinated laboratory animals. Three fractions of the anthrax protective antigen obtained possessed weaker immunobiological properties than the whole preparation of this antigen.
