Vylchev V, Syromashkova M, Mircheva I
Zh Mikrobiol Epidemiol Immunobiol. 1976 Apr(3):53-6.
An attempt was made to standardize conditions for obtaining a protective anthrax preparation. A technological scheme permitting to produce 80 mg of a purified lyophilized protective preparation with the activity of 32 immunizing units was applied. It was accepted that 1 IU was the minimal amount of the preparation which provided a survival with the mean time index of 3.0+/-0.5 of guinea pigs after a double immunization at an interval of 14 days. In vitro 1 IU also represented a minimal amount of the preparation which produced three precipitation lines (characteristic of the preparation) in immunoelectrophoretic analysis against the purified anthrax serum. Usually 1 IU in vivo and in vitro corresponded to 2.5 mg of this preparation.
人们试图规范获取保护性炭疽制剂的条件。采用了一种技术方案，该方案能够生产出80毫克活性为32个免疫单位的纯化冻干保护性制剂。规定1个国际单位（IU）是该制剂的最小量，经14天间隔的两次免疫后，能使豚鼠的平均存活时间指数为3.0±0.5。在体外，1个国际单位也代表该制剂的最小量，在针对纯化炭疽血清的免疫电泳分析中能产生三条沉淀线（该制剂的特征）。通常，体内和体外的1个国际单位相当于2.5毫克这种制剂。
